In vitro assays to evaluate CAR-T cell cytotoxicity.
Methods Cell Biol
; 183: 303-315, 2024.
Article
en En
| MEDLINE
| ID: mdl-38548415
ABSTRACT
This chapter introduces four commonly used in vitro chimeric antigen receptor (CAR)-T cell cytotoxicity assays (lactate dehydrogenase release assay, 51Cr release assay, IncuCyte live cell killing assay, and xCELLigence real-time analysis) and provides a detailed protocol for xCELLigence real-time analysis. Focusing on in vitro assays, this chapter starts with explaining the mechanisms and discussing the utilization of each assay to quantify T-cell-induced cytotoxicity. Due to the high-throughput quantification and straightforward workflow of xCELLigence real-time analysis, a protocol entailing reagents and equipment, a 3-day step-by-step procedure, and instructions for data analysis are provided.
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MEDLINE
Asunto principal:
Linfocitos T
/
Apoptosis
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En
Revista:
Methods Cell Biol
Año:
2024
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Article