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Parallel genetic screens identify nuclear envelope homeostasis as a key determinant of telomere entanglement resolution in fission yeast.
Nageshan, Rishi Kumar; Krogan, Nevan; Cooper, Julia Promisel.
Afiliación
  • Nageshan RK; Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, 12801 E. 17th Ave, Aurora, CO 80045, USA.
  • Krogan N; Former address: Telomere Biology Laboratory, Laboratory of Biochemistry and Molecular Biology, NCI, NIH, Bethesda, MD 20892, USA.
  • Cooper JP; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 1700 4th Street, 308D, San Francisco, CA 94158, USA.
G3 (Bethesda) ; 14(7)2024 Jul 08.
Article en En | MEDLINE | ID: mdl-38657142
ABSTRACT
In fission yeast lacking the telomere binding protein, Taz1, replication forks stall at telomeres, triggering deleterious downstream events. Strand invasion from one taz1Δ telomeric stalled fork to another on a separate (nonsister) chromosome leads to telomere entanglements, which are resolved in mitosis at 32°C; however, entanglement resolution fails at ≤20°C, leading to cold-specific lethality. Previously, we found that loss of the mitotic function of Rif1, a conserved DNA replication and repair factor, suppresses cold sensitivity by promoting resolution of entanglements without affecting entanglement formation. To understand the underlying pathways of mitotic entanglement resolution, we performed a series of genome-wide synthetic genetic array screens to generate a comprehensive list of genetic interactors of taz1Δ and rif1Δ. We modified a previously described screening method to ensure that the queried cells were kept in log phase growth. In addition to recapitulating previously identified genetic interactions, we find that loss of genes encoding components of the nuclear pore complex (NPC) promotes telomere disentanglement and suppresses taz1Δ cold sensitivity. We attribute this to more rapid anaphase midregion nuclear envelope (NE) breakdown in the absence of these NPC components. Loss of genes involved in lipid metabolism reverses the ability of rif1+ deletion to suppress taz1Δ cold sensitivity, again pinpointing NE modulation. A rif1+ separation-of-function mutant that specifically loses Rif1's mitotic functions yields similar genetic interactions. Genes promoting membrane fluidity were enriched in a parallel taz1+ synthetic lethal screen at permissive temperature, cementing the idea that the cold specificity of taz1Δ lethality stems from altered NE homeostasis.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Schizosaccharomyces / Telómero / Proteínas de Schizosaccharomyces pombe / Proteínas de Unión a Telómeros / Homeostasis / Membrana Nuclear Idioma: En Revista: G3 (Bethesda) Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Schizosaccharomyces / Telómero / Proteínas de Schizosaccharomyces pombe / Proteínas de Unión a Telómeros / Homeostasis / Membrana Nuclear Idioma: En Revista: G3 (Bethesda) Año: 2024 Tipo del documento: Article