Your browser doesn't support javascript.
loading
Characterization and evaluation of cytotoxic and antimicrobial activities of cyclotides from Viola japonica.
Lian, Yuanyuan; Tang, Xue; Hu, Gehui; Miao, Chenfang; Cui, Yunfei; Zhangsun, Dongting; Wu, Yong; Luo, Sulan.
Afiliación
  • Lian Y; Guangxi Key Laboratory of Special Biomedicine, School of Medicine, Guangxi University, Nanning, China.
  • Tang X; Guangxi Key Laboratory of Special Biomedicine, School of Medicine, Guangxi University, Nanning, China.
  • Hu G; Guangxi Key Laboratory of Special Biomedicine, School of Medicine, Guangxi University, Nanning, China.
  • Miao C; Department of Pharmacy, The 900Th Hospital of Joint Logistics Team of the PLA, Fuzhou General Clinical Medical College of Fujian Medical University, Fuzhou, China.
  • Cui Y; Guangxi Key Laboratory of Special Biomedicine, School of Medicine, Guangxi University, Nanning, China.
  • Zhangsun D; Guangxi Key Laboratory of Special Biomedicine, School of Medicine, Guangxi University, Nanning, China.
  • Wu Y; Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, China.
  • Luo S; Guangxi Key Laboratory of Special Biomedicine, School of Medicine, Guangxi University, Nanning, China. wuyong@gxu.edu.cn.
Sci Rep ; 14(1): 9733, 2024 04 28.
Article en En | MEDLINE | ID: mdl-38679643
ABSTRACT
Cyclotides are a type of defense peptide most commonly found in the Violaceae family of plants, exhibiting various biological activities. In this study, we focused on the Viola japonica as our research subject and conducted transcriptome sequencing and analysis using high-throughput transcriptomics techniques. During this process, we identified 61 cyclotides, among which 25 were previously documented, while the remaining 36 were designated as vija 1 to vija 36. Mass spectrometry detection showed that 21 putative cyclotides were found in the extract of V. japonica. Through isolation, purification and tandem mass spectrometry, we characterized and investigated the activities of five cyclotides. Our results demonstrated inhibitory effects of these cyclotides on the growth of Acinetobacter baumannii and Bacillus subtilis, with minimum inhibitory concentrations (MICs) of 4.2 µM and 2.1 µM, respectively. Furthermore, time killing kinetic assays revealed that cyclotides at concentration of 4 MICs achieved completely bactericidal effects within 2 h. Additionally, fluorescence staining experiments confirmed that cyclotides disrupt microbial membranes. Moreover, cytotoxicity studies showed that cyclotides possess cytotoxic effects, with IC50 values ranging from 0.1 to 3.5 µM. In summary, the discovery of new cyclotide sequences enhances our understanding of peptide diversity and the exploration of their activity lays the foundation for a deeper investigation into the mechanisms of action of cyclotides.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Pruebas de Sensibilidad Microbiana / Acinetobacter baumannii / Viola / Ciclotidas Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Pruebas de Sensibilidad Microbiana / Acinetobacter baumannii / Viola / Ciclotidas Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article