Synthesis, characterization, and investigation of antibacterial activity of Novel CMC/CuO NPs/CQDs bionanocomposite coating.

ABSTRACT

In recent decades, healthcare-associated infections (HAIs) have become a common problem in healthcare facilities such as hospitals. As a result, researchers are currently developing nanocomposite coatings that are strengthened with antibacterial nanoparticles. In this research, a novel antibacterial bionanocomposite coating based on carboxymethyl cellulose polymer/copper oxide nanoparticles/carbon quantum dots was coated on medical grade 316 stainless steel by sol-gel dip-coating method. The effect of the concentration of nanocomposite components was investigated at four different levels to determine the best ratio with the most antibacterial activity. Structural characteristics of nanocomposite and coating were investigated using different analysis methods. The coating analysis showed that reinforcements are uniformly distributed in the polymer matrix. Antibacterial test of disc diffusion was performed by the Kirby-Bauer method and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial test. The results showed that bionanocomposite was effective in the MIC assays against Staphylococcus aureus and Escherichia coli with MIC values of 25 mg/ml and >50 mg/ml, respectively. The inhibition zones for E. coli and S. aureus were 17 and 32 mm, respectively, at 10 µg/disc of gentamicin. SEM images displayed significant and evident alterations in the structure of bacterial morphology, indicating cellular damage.