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On the compatibility of single-cell microcarriers (nanovials) with microfluidic impedance cytometry.
Brandi, Cristian; De Ninno, Adele; Ruggiero, Filippo; Limiti, Emanuele; Abbruzzese, Franca; Trombetta, Marcella; Rainer, Alberto; Bisegna, Paolo; Caselli, Federica.
Afiliación
  • Brandi C; Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Rome, Italy. caselli@ing.uniroma2.it.
  • De Ninno A; Italian National Research Council - Institute for Photonics and Nanotechnologies (CNR - IFN), Rome, Italy.
  • Ruggiero F; Italian National Research Council - Institute for Photonics and Nanotechnologies (CNR - IFN), Rome, Italy.
  • Limiti E; Department of Engineering, Università Campus Bio-Medico di Roma, Via Álvaro del Portillo 21, 00128, Rome, Italy.
  • Abbruzzese F; Department of Engineering, Università Campus Bio-Medico di Roma, Via Álvaro del Portillo 21, 00128, Rome, Italy.
  • Trombetta M; Department of Science and Technology for Sustainable Development and One Health, Università Campus Bio-Medico di Roma, Via Álvaro del Portillo 21, 00128, Rome, Italy.
  • Rainer A; Department of Engineering, Università Campus Bio-Medico di Roma, Via Álvaro del Portillo 21, 00128, Rome, Italy.
  • Bisegna P; National Research Council - Institute of Nanotechnology (CNR-NANOTEC), c/o Campus Ecotekne, 73100 Lecce, Italy.
  • Caselli F; Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Rome, Italy. caselli@ing.uniroma2.it.
Lab Chip ; 24(11): 2883-2892, 2024 May 28.
Article en En | MEDLINE | ID: mdl-38717432
ABSTRACT
We investigate for the first time the compatibility of nanovials with microfluidic impedance cytometry (MIC). Nanovials are suspendable crescent-shaped single-cell microcarriers that enable specific cell adhesion, the creation of compartments for undisturbed cell growth and secretion, as well as protection against wall shear stress. MIC is a label-free single-cell technique that characterizes flowing cells based on their electrical fingerprints and it is especially targeted to cells that are naturally in suspension. Combining nanovial technology with MIC is intriguing as it would represent a robust framework for the electrical analysis of single adherent cells at high throughput. Here, as a proof-of-concept, we report the MIC analysis of mesenchymal stromal cells loaded in nanovials. The electrical analysis is supported by numerical simulations and validated by means of optical analysis. We demonstrate that the electrical diameter can discriminate among free cells, empty nanovials, cell-loaded nanovials, and clusters, thus grounding the foundation for the use of nanovials in MIC. Furthermore, we investigate the potentiality of MIC to assess the electrical phenotype of cells loaded in nanovials and we draw directions for future studies.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Técnicas Analíticas Microfluídicas / Análisis de la Célula Individual / Células Madre Mesenquimatosas Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Técnicas Analíticas Microfluídicas / Análisis de la Célula Individual / Células Madre Mesenquimatosas Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article