Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of GFAP in serum.
Mikrochim Acta
; 191(6): 325, 2024 05 13.
Article
en En
| MEDLINE
| ID: mdl-38739279
ABSTRACT
Glial fibrillary acidic protein (GFAP) in serum has been shown as a biomarker of traumatic brain injury (TBI) which is a significant global public health concern. Accurate and rapid detection of serum GFAP is critical for TBI diagnosis. In this study, a time-resolved fluorescence immunochromatographic test strip (TRFIS) was proposed for the quantitative detection of serum GFAP. This TRFIS possessed excellent linearity ranging from 0.05 to 2.5 ng/mL for the detection of serum GFAP and displayed good linearity (Y = 598723X + 797198, R2 = 0.99), with the lowest detection limit of 16 pg/mL. This TRFIS allowed for quantitative detection of serum GFAP within 15 min and showed high specificity. The intra-batch coefficient of variation (CV) and the inter-batch CV were both < 4.0%. Additionally, this TRFIS was applied to detect GFAP in the serum samples from healthy donors and patients with cerebral hemorrhage, and the results of TRFIS could efficiently discern the patients with cerebral hemorrhage from the healthy donors. Our developed TRFIS has the characteristics of high sensitivity, high accuracy, and a wide linear range and is suitable for rapid and quantitative determination of serum GFAP on-site.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Cromatografía de Afinidad
/
Proteína Ácida Fibrilar de la Glía
Idioma:
En
Revista:
Mikrochim Acta
Año:
2024
Tipo del documento:
Article