Genetic Transformation of Cryptococcus Species with Agrobacterium Transfer DNA.

Chong, Nicholas F; Idnurm, Alexander; Nugent, Bridgit C

Chong, Nicholas F; Idnurm, Alexander; Nugent, Bridgit C.

Afiliación

Chong NF; School of BioSciences, The University of Melbourne, Parkville, VIC, Australia.
Idnurm A; School of BioSciences, The University of Melbourne, Parkville, VIC, Australia. alexander.idnurm@unimelb.edu.au.
Nugent BC; School of BioSciences, The University of Melbourne, Parkville, VIC, Australia.

Methods Mol Biol ; 2775: 81-90, 2024.

Article en En | MEDLINE | ID: mdl-38758312

ABSTRACT

ABSTRACT

Transformation of foreign DNA into Cryptococcus species is a powerful tool for exploring gene functions in these human pathogens. Agrobacterium tumefaciens-mediated transformation (AtMT) has been used for the stable introduction of exogenous DNA into Cryptococcus for over two decades, being particularly impactful for insertional mutagenesis screens to discover new genes involved in fungal biology. A detailed protocol to conduct this transformation method is provided in the chapter. Scope for modifications and the benefits and disadvantages of using AtMT in Cryptococcus species are also presented.

Asunto(s)

Agrobacterium tumefaciens; Cryptococcus; Transformación Genética; Cryptococcus/genética; Agrobacterium tumefaciens/genética; ADN Bacteriano/genética; Vectores Genéticos/genética; Técnicas de Transferencia de Gen

Palabras clave

Agrobacterium; Complementation; Cryptococcus; Forward genetics; Genetic modification; Mutant screens

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Transformación Genética / Agrobacterium tumefaciens / Cryptococcus Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article

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