Cu superparticle-based aggregation induced enhancement strategy with PVDF-HFP/CeVO4 NP sensing interface for miR-103a detection.
Talanta
; 276: 126289, 2024 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-38776779
ABSTRACT
Aggregation-induced emission (AIE) has been widely used in research on electrochemiluminescence (ECL) due to its excellent luminescence intensity. In this work, copper superparticles (Cu SPs) were used to construct ECL biosensor to detect the microRNA-103a (miRNA-103a) in triple-negative breast cancer (TNBC) tumor tissues. Firstly, GSH-capped copper clusters were used as precursors to prepare Cu SPs by the AIE effect. Compared with clusters, Cu SPs possessed higher luminescence performance and energy stability, making them an ideal choice for ECL nanoprobe. The film of PVDF-HFP/CeVO4 NPs was constructed and modified with CPBA and GSH as the sensing interface (PCCG). The PCCG film displayed good conductivity and hydrophilicity, and desirable mechanical stability. Moreover, the PCCG film can induce high carrier mobility rates and dissociate large amounts of the co-reactant K2S2O8 to enhance the ECL intensity of Cu SPs. As a result, the prepared ECL sensor with the catalyzed hairpin assembly (CHA) strategy was employed to quantify miRNA-103a in the range of 100 fM to 100 nM. The biosensor provided a novel analytical approach for the clinical diagnosis of TNBC.
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Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
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Cobre
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MicroARNs
Idioma:
En
Revista:
Talanta
Año:
2024
Tipo del documento:
Article