CircMYBL2 facilitates hepatocellular carcinoma progression by regulating E2F1 expression.
Oncol Res
; 32(6): 1129-1139, 2024.
Article
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| MEDLINE
| ID: mdl-38827325
ABSTRACT
Circular RNAs (circRNAs) have been recognized as pivotal regulators in tumorigenesis, yet the biological functions as well as molecular mechanisms of the majority of circRNAs in hepatocellular carcinoma (HCC) remain elusive. We sought to unveil the expression profile and biological role of circMYBL2 in HCC. Initial microarray analyses were conducted to probe the expression profile of circMYBL2 in HCC cells, and qRTâPCR analysis was then performed in HCC cell lines and tissues, revealing significant upregulation of circMYBL2. Subsequent experiments were conducted to evaluate the biological function of circMYBL2 in HCC progression. Furthermore, bioinformatics analysis, qRTâPCR analysis, luciferase reporter assays, and western blot analysis were employed to investigate the interplay among circMYBL2, miR-1205, and E2F1. CircMYBL2 was found to exhibit marked upregulation in tumor tissues as well as HCC cell lines. Elevated expression of circMYBL2 increased the proliferation and migration of HCC cells, whereas circMYBL2 knockdown elicited contrasting effects. Mechanistically, our results indicated that circMYBL2 promoted E2F1 expression and facilitated HCC progression by sponging miR-1205. Our findings revealed that circMYBL2 contributed to HCC progression through the circMYBL2/miR-1205/E2F1 axis, suggesting the potential of circMYBL2 as a novel target for HCC treatment or a prognostic biomarker for HCC.
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Base de datos:
MEDLINE
Asunto principal:
Regulación Neoplásica de la Expresión Génica
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Carcinoma Hepatocelular
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Progresión de la Enfermedad
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Factor de Transcripción E2F1
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ARN Circular
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Neoplasias Hepáticas
Idioma:
En
Revista:
Oncol Res
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Oncol. res
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Oncology research
Asunto de la revista:
NEOPLASIAS
Año:
2024
Tipo del documento:
Article