Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
STAR Protoc
; 5(2): 103111, 2024 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-38833372
ABSTRACT
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic "livers in a dish." We describe strategies to induce metabolic and alcohol-associated hepatic steatosis, inflammation, and fibrosis. We outline potential applications, including using human liver spheroids for experimental and translational research and drug screening to identify potential anti-fibrotic therapies.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Esferoides Celulares
/
Hígado
/
Cirrosis Hepática
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article