Multi-regional sequencing reveals the genetic and immune heterogeneity of non-cancerous tissues in gastric cancer.
J Pathol
; 263(4-5): 454-465, 2024 Aug.
Article
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| MEDLINE
| ID: mdl-38845115
ABSTRACT
Gastric cancer (GC) is one of the most heterogeneous tumors. However, research on normal tissue adjacent to the tumor (NAT) is very limited. We performed multi-regional omics sequencing on 150 samples to assess the genetic basis and immune microenvironment in NAT and matched primary tumor or lymph node metastases. NATs demonstrated different mutated genes compared with GC, and NAT genomes underwent independent evolution with low variant allele frequency. Mutation profiles were predominated by aging and smoking-associated signatures in NAT instead of signatures associated with genetic instability. Although the immune microenvironment within NATs shows substantial intra-patient heterogeneity, the proportion of shared TCR clones among NATs is five times higher than that of tumor regions. These findings support the notion that subclonal expansion is not pronounced in NATs. We also demonstrated remarkable intra-patient heterogeneity of GCs and revealed heterogeneity of focal amplification of CD274 (encoding PD-L1) that leads to differential expression. Finally, we identified that monoclonal seeding is predominant in GC, which is followed by metastasis-to-metastasis dissemination in individual lymph nodes. These results provide novel insights into GC carcinogenesis. © 2024 The Pathological Society of Great Britain and Ireland.
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MEDLINE
Asunto principal:
Neoplasias Gástricas
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Microambiente Tumoral
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Antígeno B7-H1
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Mutación
Idioma:
En
Revista:
J Pathol
Año:
2024
Tipo del documento:
Article