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An improved genome assembly of Chrysanthemum nankingense reveals expansion and functional diversification of terpene synthase gene family.
Jiang, Liping; Chen, Shi; Wang, Xu; Sen, Lin; Dong, Gangqiang; Song, Chi; Liu, Yifei.
Afiliación
  • Jiang L; Department of Pharmacy, Wuhan No.1 Hospital (Wuhan Hospital of Traditional and Western Medicine), Wuhan, 430022, People's Republic of China.
  • Chen S; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, People's Republic of China.
  • Wang X; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, People's Republic of China.
  • Sen L; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, People's Republic of China.
  • Dong G; Amway (China) Botanical R&D Center, Wuxi, 214115, P.R. China.
  • Song C; Institute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu, 610000, People's Republic of China. songchi@cdutcm.edu.cn.
  • Liu Y; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, People's Republic of China. liuyifei@hbtcm.edu.cn.
BMC Genomics ; 25(1): 593, 2024 Jun 12.
Article en En | MEDLINE | ID: mdl-38867153
ABSTRACT

BACKGROUND:

Terpenes are important components of plant aromas, and terpene synthases (TPSs) are the key enzymes driving terpene diversification. In this study, we characterized the volatile terpenes in five different Chrysanthemum nankingense tissues. In addition, genome-wide identification and expression analysis of TPS genes was conducted utilizing an improved chromosome-scale genome assembly and tissue-specific transcriptomes. The biochemical functions of three representative TPSs were also investigated.

RESULTS:

We identified tissue-specific volatile organic compound (VOC) and volatile terpene profiles. The improved Chrysanthemum nankingense genome assembly was high-quality, including a larger assembled size (3.26 Gb) and a better contig N50 length (3.18 Mb) compared to the old version. A total of 140 CnTPS genes were identified, with the majority representing the TPS-a and TPS-b subfamilies. The chromosomal distribution of these TPS genes was uneven, and 26 genes were included in biosynthetic gene clusters. Closely-related Chrysanthemum taxa were also found to contain diverse TPS genes, and the expression profiles of most CnTPSs were tissue-specific. The three investigated CnTPS enzymes exhibited versatile activities, suggesting multifunctionality.

CONCLUSIONS:

We systematically characterized the structure and diversity of TPS genes across the Chrysanthemum nankingense genome, as well as the potential biochemical functions of representative genes. Our results provide a basis for future studies of terpene biosynthesis in chrysanthemums, as well as for the breeding of improved chrysanthemum varieties.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Terpenos / Familia de Multigenes / Genoma de Planta / Transferasas Alquil y Aril / Chrysanthemum Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Terpenos / Familia de Multigenes / Genoma de Planta / Transferasas Alquil y Aril / Chrysanthemum Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article