Extended depth-of-field wide-field fluorescence microscopy with a micro-mirror array lens system for versatile cellular examination.
Opt Lett
; 49(12): 3368-3371, 2024 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-38875622
ABSTRACT
We present a versatile extended depth-of-field (EDOF) wide-field fluorescence microscopy using a new, to the best of our knowledge, active device, micro-mirror array lens system (MALS) for calibration-free and orientation-insensitive EDOF imaging. The MALS changed the focal plane during image acquisition, and the system could be operated in any orientation. Two EDOF imaging modes of high-speed accumulation and low-speed surface sectioning were implemented. The performance was demonstrated in non-contact imaging of conjunctival goblet cells in live mice and depth-resolved cellular examination of ex-vivo human cancer specimens. MALS-based EDOF microscopy has potential for versatile cellular examination.
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MEDLINE
Asunto principal:
Lentes
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Microscopía Fluorescente
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En
Revista:
Opt Lett
Año:
2024
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Article