Superoxide is an Intrinsic Signaling Molecule Triggering Muscle Hypertrophy.
Antioxid Redox Signal
; 2024 Jul 12.
Article
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| MEDLINE
| ID: mdl-38877802
ABSTRACT
Aims:
Redox signaling plays a key role in skeletal muscle remodeling induced by exercise and prolonged inactivity, but it is unclear which oxidant triggers myofiber hypertrophy due to the lack of strategies to precisely regulate individual oxidants in vivo. In this study, we used tetrathiomolybdate (TM) to dissociate the link between superoxide (O2â¢-) and hydrogen peroxide and thereby to specifically explore the role of O2â¢- in muscle hypertrophy in C2C12 cells and mice.Results:
TM can linearly regulate intracellular O2â¢- levels by inhibition of superoxide dismutase 1 (SOD1). A 70% increase in O2â¢- levels in C2C12 myoblast cells and mice is necessary and sufficient for triggering hypertrophy of differentiated myotubes and can enhance exercise performance by more than 50% in mice. SOD1 knockout blocks TM-induced O2â¢- increments and thereby prevents hypertrophy, whereas SOD1 restoration rescues all these effects. Scavenging O2â¢- with antioxidants abolishes TM-induced hypertrophy and the enhancement of exercise performance, whereas the restoration of O2â¢- levels with a O2â¢- generator promotes muscle hypertrophy independent of SOD1 activity. Innovation andConclusion:
These findings suggest that O2â¢- is an endogenous initiator of myofiber hypertrophy and that TM may be used to treat muscle wasting diseases. Our work not only suggests a novel druggable mechanism to increase muscle mass but also provides a tool for precisely regulating O2â¢- levels in vivo.
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Base de datos:
MEDLINE
Idioma:
En
Revista:
Antioxid Redox Signal
Asunto de la revista:
METABOLISMO
Año:
2024
Tipo del documento:
Article