A new procedure to induce aortic aneurysms in mice.
Methods Cell Biol
; 188: 61-71, 2024.
Article
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| MEDLINE
| ID: mdl-38880528
ABSTRACT
Aortic aneurysms (AAs) are a major public health challenge, featured by a progressive impairs in aortic wall integrity that drives to aortic dilation and, in end stage, to its rupture. Despite important advances in the surgical treatment of aortic aneurysms, there is currently no pharmacological intervention that prevents their development, reduces their expansion, or avoids their rupture. In addition to classic risk factors such age or gender, several heritable connective tissue disorders have been associated with AA developing, highlighting the role of extracellular matrix (ECM) genes alterations in the developing of AA. In this sense, we have recently demonstrated that global deletion of the cellular communicating network factor 2 (CCN2), previously known as connective tissue growth factor (CTGF) due to its role in the extracellular matrix formation, predisposes to early and lethal AAs development after Angiotensin II (Ang II) infusion in mice. Here, we detail the protocol to induce and detect AAs generation in inducible global CCN2 knockout mice after Ang II infusion which allow the characterization of CCN role in AA development and may help to the development of pharmacological target for AA treatment.
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Base de datos:
MEDLINE
Asunto principal:
Aneurisma de la Aorta
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Angiotensina II
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Ratones Noqueados
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Modelos Animales de Enfermedad
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Factor de Crecimiento del Tejido Conjuntivo
Idioma:
En
Revista:
Methods Cell Biol
Año:
2024
Tipo del documento:
Article