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Employing Genomic Tools to Explore the Molecular Mechanisms behind the Enhancement of Plant Growth and Stress Resilience Facilitated by a Burkholderia Rhizobacterial Strain.
Chang, Yueh-Long; Chang, Yu-Cheng; Kurniawan, Andi; Chang, Po-Chun; Liou, Ting-Yu; Wang, Wen-Der; Chuang, Huey-Wen.
Afiliación
  • Chang YL; Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan.
  • Chang YC; Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan.
  • Kurniawan A; Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan.
  • Chang PC; Department of Agronomy, Brawijaya University, Malang 65145, Indonesia.
  • Liou TY; Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan.
  • Wang WD; Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan.
  • Chuang HW; Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan.
Int J Mol Sci ; 25(11)2024 May 31.
Article en En | MEDLINE | ID: mdl-38892282
ABSTRACT
The rhizobacterial strain BJ3 showed 16S rDNA sequence similarity to species within the Burkholderia genus. Its complete genome sequence revealed a 97% match with Burkholderia contaminans and uncovered gene clusters essential for plant-growth-promoting traits (PGPTs). These clusters include genes responsible for producing indole acetic acid (IAA), osmolytes, non-ribosomal peptides (NRPS), volatile organic compounds (VOCs), siderophores, lipopolysaccharides, hydrolytic enzymes, and spermidine. Additionally, the genome contains genes for nitrogen fixation and phosphate solubilization, as well as a gene encoding 1-aminocyclopropane-1-carboxylate (ACC) deaminase. The treatment with BJ3 enhanced root architecture, boosted vegetative growth, and accelerated early flowering in Arabidopsis. Treated seedlings also showed increased lignin production and antioxidant capabilities, as well as notably increased tolerance to water deficit and high salinity. An RNA-seq transcriptome analysis indicated that BJ3 treatment significantly activated genes related to immunity induction, hormone signaling, and vegetative growth. It specifically activated genes involved in the production of auxin, ethylene, and salicylic acid (SA), as well as genes involved in the synthesis of defense compounds like glucosinolates, camalexin, and terpenoids. The expression of AP2/ERF transcription factors was markedly increased. These findings highlight BJ3's potential to produce various bioactive metabolites and its ability to activate auxin, ethylene, and SA signaling in Arabidopsis, positioning it as a new Burkholderia strain that could significantly improve plant growth, stress resilience, and immune function.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Arabidopsis / Burkholderia Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Arabidopsis / Burkholderia Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article