Quantification of thermodynamic effects of carbohydrate multivalency on avidity using synthetic discrete glycooligomers.
Chem Commun (Camb)
; 60(55): 7021-7024, 2024 Jul 04.
Article
en En
| MEDLINE
| ID: mdl-38895769
ABSTRACT
A quantitative understanding of thermodynamic effects of avidity in biomolecular interactions is important. Herein, we synthesized discrete glycooligomers and evaluated their interactions with a model protein using isothermal titration calorimetry. The dimeric glycooligomer exhibited higher binding constants compared to the glycomonomer, attributed to the reduced conformational entropy loss through local presentation of multiple carbohydrate units. Conversely, divalent glycoligands with polyethylene glycol linkers, aiming for multivalent binding, showed enhanced interactions through increased enthalpy. These findings emphasize the importance of distinguishing between the "local avidity" and the "multipoint avidity".
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Base de datos:
MEDLINE
Asunto principal:
Termodinámica
Idioma:
En
Revista:
Chem Commun (Camb)
Asunto de la revista:
QUIMICA
Año:
2024
Tipo del documento:
Article