Natural Prenylflavonoids from Sophora flavescens Root Bark against Multidrug-Resistant Methicillin-Sensitive Staphylococcus aureus Targeting the Membrane Permeability.
J Agric Food Chem
; 72(26): 14684-14700, 2024 Jul 03.
Article
en En
| MEDLINE
| ID: mdl-38905352
ABSTRACT
The overuse of antibiotics in animal farming and aquaculture has led to multidrug-resistant methicillin-sensitive Staphylococcus aureus (MR-MSSA) becoming a common pathogen in foodborne diseases. Sophora flavescens Ait. serves as a traditional plant antibacterial agent and functional food ingredient. A total of 30 compounds (1-30) were isolated from the root bark of S. flavescens, consisting of 20 new compounds (1-20). In the biological activity assay, compound 1 demonstrated a remarkable inhibitory effect on MR-MSSA, with an MIC of 2 µg/mL. Furthermore, 1 was found to rapidly eliminate bacteria, inhibit biofilm growth, and exhibit exceptionally low cytotoxicity. Mechanistic studies have revealed that 1 possesses an enhanced membrane-targeting ability, binding to the bacterial cell membrane components phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and cardiolipin (CL). This disruption of bacterial cell membrane integrity increases intracellular reactive oxygen species, protein and DNA leakage, reduced bacterial metabolism, and ultimately bacterial death. In summary, these findings suggest that compound 1 holds promise as a lead compound against MR-MSSA.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Flavonoides
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Extractos Vegetales
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Pruebas de Sensibilidad Microbiana
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Permeabilidad de la Membrana Celular
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Raíces de Plantas
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Sophora
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Corteza de la Planta
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Antibacterianos
Idioma:
En
Revista:
J Agric Food Chem
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J. agric. Food chem
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Journal of agricultural and food chemistry
Año:
2024
Tipo del documento:
Article