Single-cell transcriptomic profiling unveils insights into ovarian fibrosis in obese mice.
Biol Direct
; 19(1): 52, 2024 Jul 02.
Article
en En
| MEDLINE
| ID: mdl-38956667
ABSTRACT
BACKGROUND:
Adiposity profoundly impacts reproductive health in both humans and animals. However, the precise subpopulations contributing to infertility under obese conditions remain elusive.RESULTS:
In this study, we established an obese mouse model through an eighteen-week high-fat diet regimen in adult female mice. Employing single-cell RNA sequencing (scRNA-seq), we constructed a comprehensive single-cell atlas of ovarian tissues from these mice to scrutinize the impact of obesity on the ovarian microenvironment. ScRNA-seq revealed notable alterations in the microenvironment of ovarian tissues in obese mice. Granulosa cells, stromal cells, T cells, and macrophages exhibited functional imbalances compared to the control group. We observed heightened interaction strength in the SPP1-CD44 pairing within lgfbp7+ granulosa cell subtypes and Il1bhigh monocyte subtypes in the ovarian tissues of obese mice. Moreover, the interaction strength between Il1bhigh monocyte subtypes and Pdgfrb+ stromal cell subtypes in the form of TNF - TNFrsf1α interaction was also enhanced subsequently to obesity, potentially contributing to ovarian fibrosis pathogenesis.CONCLUSIONS:
We propose a model wherein granulosa cells secrete SPP1 to activate monocytes, subsequently triggering TNF-α secretion by monocytes, thereby activating stromal cells and ultimately leading to the development of ovarian fibrosis. Intervening in this process may represent a promising avenue for improving clinical outcomes in fertility treatments for obese women.Palabras clave
Texto completo:
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Base de datos:
MEDLINE
Asunto principal:
Fibrosis
/
Análisis de la Célula Individual
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Ratones Obesos
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Obesidad
Idioma:
En
Revista:
Biol Direct
Año:
2024
Tipo del documento:
Article