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Establishment and characterization of cMYB-expressing human salivary adenoid cystic carcinoma cell lines (UM-HACC-14, UM-HACC-6) and matching patient-derived xenograft model (UM-PDX-HACC-14).
Warner, Kristy A; Herzog, Alexandra E; Sahara, Sosuke; Nör, Felipe; Castilho, Rogerio M; Demirci, Hakan; Chepeha, Douglas B; Polverini, Peter J; Nör, Jacques E.
Afiliación
  • Warner KA; Department of Cariology, Restorative Sciences, Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
  • Herzog AE; Department of Cariology, Restorative Sciences, Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
  • Sahara S; Department of Cariology, Restorative Sciences, Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
  • Nör F; Department of Periodontics and Oral Medicine, School of Dentistry, Ann Arbor, MI, USA.
  • Castilho RM; Department of Periodontics and Oral Medicine, School of Dentistry, Ann Arbor, MI, USA.
  • Demirci H; Department of Opthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan, Ann Arbor, MI, USA.
  • Chepeha DB; Department of Otolaryngology, University of Toronto, Toronto, ON, Canada.
  • Polverini PJ; Department of Otolaryngology, University of Michigan School of Medicine, Ann Arbor, MI, USA; Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI, USA; Department of Periodontics and Oral Medicine, School of Dentistry, Ann Arbor, MI, USA; Department of Pathology, University of Michigan
  • Nör JE; Department of Cariology, Restorative Sciences, Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, MI, USA; Department of Otolaryngology, University of Michigan School of Medicine
Oral Surg Oral Med Oral Pathol Oral Radiol ; 2024 Jun 11.
Article en En | MEDLINE | ID: mdl-38971694
ABSTRACT

OBJECTIVE:

Limited availability of authentic human adenoid cystic carcinoma (ACC) cell lines has hindered progress in understanding mechanisms underpinning the biology of this disease and the development of safe and effective therapies. STUDY

DESIGN:

Surgical human ACC specimens (UM-HACC-6, UM-HACC-14) were dissociated into single cell suspensions and cultured in fibronectin-coated flasks. Alternatively, tumor fragments were transplanted subcutaneously into female immunodeficient (SCID) mice to establish patient-derived xenograft tumors (PDX; UM-PDX-HACC-14).

RESULTS:

Both ACC cell lines showed continuous growth in monolayers for over 100 passages. Total RNA-Seq, RT-PCR, and FISH analysis revealed that both are MYB-NFIB fusion negative. Western blots revealed passage-dependent expression of E-Cadherin, PCNA, p63, phospho-c-MYB, and NFIB. Both, UM-HACC-14 and UM-HACC-6 cells exhibited tumorigenic potential when injected orthotopically into mouse submandibular glands.

CONCLUSION:

UM-HACC-14, patient-matching UM-PDX-HACC-14, and the UM-HACC-6 cell line are new, authenticated preclinical models of ACC that are well suited for mechanistic and developmental therapeutics studies.
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Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Oral Surg Oral Med Oral Pathol Oral Radiol Año: 2024 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Oral Surg Oral Med Oral Pathol Oral Radiol Año: 2024 Tipo del documento: Article