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STED Imaging of Vesicular Endocytosis in the Synapse.
Hu, Shaoqin; Xie, Zhenli; Wang, Bianbian; Chen, Yang; Jing, Zexin; Hao, Ying; Yao, Jingyu; Wu, Xuanang; Huo, Jingxiao; Wei, Anqi; Qin, Yuhao; Dong, Nan; Zheng, Chaowen; Song, Qian; Long, Jiangang; Kang, Xinjiang; Wang, Changhe; Xu, Huadong.
Afiliación
  • Hu S; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Xie Z; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Wang B; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Chen Y; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Jing Z; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Hao Y; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Yao J; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Wu X; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Huo J; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Wei A; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Qin Y; Key Laboratory of Medical Electrophysiology, Ministry of Education of China, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, and the Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China.
  • Dong N; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Zheng C; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Song Q; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Long J; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
  • Kang X; College of Life Sciences, Liaocheng University, Liaocheng, 252059, China. kxj335@163.com.
  • Wang C; Key Laboratory of Medical Electrophysiology, Ministry of Education of China, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, and the Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China. kxj335@163.com.
  • Xu H; Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China. changhewang@x
Neurosci Bull ; 2024 Jul 08.
Article en En | MEDLINE | ID: mdl-38976218
ABSTRACT
Endocytosis is a fundamental biological process that couples exocytosis to maintain the homeostasis of the plasma membrane and sustained neurotransmission. Super-resolution microscopy enables optical imaging of exocytosis and endocytosis in live cells and makes an essential contribution to understanding molecular mechanisms of endocytosis in neuronal somata and other types of cells. However, visualization of exo-endocytic events at the single vesicular level in a synapse with optical imaging remains a great challenge to reveal mechanisms governing the synaptic exo-endocytotic coupling. In this protocol, we describe the technical details of stimulated emission depletion (STED) imaging of synaptic endocytosis at the single-vesicle level, from sample preparation and microscopy calibration to data acquisition and analysis.
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Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Neurosci Bull Asunto de la revista: NEUROLOGIA Año: 2024 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Neurosci Bull Asunto de la revista: NEUROLOGIA Año: 2024 Tipo del documento: Article