Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat.
Methods Mol Biol
; 2830: 131-136, 2024.
Article
en En
| MEDLINE
| ID: mdl-38977574
ABSTRACT
Seed dormancy genes typically suppress germination and cell division. Therefore, overexpressing these genes can negatively affect tissue culture, interfering with the generation of transgenic plants and thus hampering the analysis of gene function. Transient expression in target cells is a useful approach for studying the function of seed dormancy genes. Here, we describe a protocol for transiently expressing genes related to seed dormancy in the scutellum of immature wheat (Triticum aestivum) embryos to analyze their effects on germination.
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1
Base de datos:
MEDLINE
Asunto principal:
Semillas
/
Triticum
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Germinación
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Regulación de la Expresión Génica de las Plantas
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Latencia en las Plantas
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article