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Analysis of Antioxidant Compounds in Vitex negundo Leaves Using Offline 2D-LC-ECD and LC-MS/MS.
Wu, Qimei; Zheng, Jinfen; Yu, Yan; Li, Zhirong; Li, Ying; Hu, Chengfeng; Zhou, Yaping; Chen, Rongxiang.
Afiliación
  • Wu Q; School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
  • Zheng J; School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
  • Yu Y; School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
  • Li Z; School of Basic Medicine, Zunyi Medical University, Zunyi 563000, China.
  • Li Y; School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
  • Hu C; School of Basic Medicine, Zunyi Medical University, Zunyi 563000, China.
  • Zhou Y; School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
  • Chen R; School of Basic Medicine, Zunyi Medical University, Zunyi 563000, China.
Molecules ; 29(13)2024 Jul 01.
Article en En | MEDLINE | ID: mdl-38999085
ABSTRACT
Vitex negundo has strong antioxidant activity, but its primary antioxidant components are not clear. In this study, the antioxidant components were screened by offline two-dimensional liquid chromatography coupled with electrochemical detection (2D-LC-ECD) and subsequently assessed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification, radical scavenging capacity, and molecular docking. Various fractions were isolated from Vitex negundo leaves, and 39 antioxidant components were screened and identified. All of the fractions containing the antioxidant components exhibited certain antioxidant activity. Correlation analysis revealed a strong correlation between the response of LC-ECD and the in vitro antioxidant activity of the fractions. Molecular docking demonstrated that components with high response to LC-ECD exhibited robust interaction with antioxidant-related target proteins. The main antioxidant components of Vitex negundo leaves were isoorientin, chlorogenic acid, agnuside, cynaroside, and scutellarin. The 2D-LC-ECD combined with LC-MS/MS was rapid and effective in screening the antioxidant components in Vitex negundo leaves and could also provide technical support for the discovery of antioxidant components with different polarities and contents in other medicinal and edible plants.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Hojas de la Planta / Vitex / Espectrometría de Masas en Tándem / Simulación del Acoplamiento Molecular / Antioxidantes Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Hojas de la Planta / Vitex / Espectrometría de Masas en Tándem / Simulación del Acoplamiento Molecular / Antioxidantes Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article