Tandem LIM domain-containing proteins, LIMK1 and LMO1, directly bind to force-bearing keratin intermediate filaments.
Cell Rep
; 43(7): 114480, 2024 Jul 23.
Article
en En
| MEDLINE
| ID: mdl-39003737
ABSTRACT
The cytoskeleton of the cell is constantly exposed to physical forces that regulate cellular functions. Selected members of the LIM (Lin-11, Isl-1, and Mec-3) domain-containing protein family accumulate along force-bearing actin fibers, with evidence supporting that the LIM domain is solely responsible for this force-induced interaction. However, LIM domain's force-induced interactions are not limited to actin. LIMK1 and LMO1, both containing only two tandem LIM domains, are recruited to force-bearing keratin fibers in epithelial cells. This unique recruitment is mediated by their LIM domains and regulated by the sequences outside the LIM domains. Based on in vitro reconstitution of this interaction, LIMK1 and LMO1 directly interact with stretched keratin 8/18 fibers. These results show that LIM domain's mechano-sensing abilities extend to the keratin cytoskeleton, highlighting the diverse role of LIM proteins in force-regulated signaling.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Filamentos Intermedios
/
Quinasas Lim
/
Proteínas con Dominio LIM
/
Queratinas
Idioma:
En
Revista:
Cell Rep
Año:
2024
Tipo del documento:
Article