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Novel insights into paclitaxel's role on tumor-associated macrophages in enhancing PD-1 blockade in breast cancer treatment.
Choi, Yoonjeong; Kim, Seong A; Jung, Hanul; Kim, Eunhae; Kim, Yoon Kyoung; Kim, Seohyun; Kim, Jaehyun; Lee, Yeji; Jo, Min Kyoung; Woo, Jiwan; Cho, Yakdol; Lee, Dongjoo; Choi, Hongyoon; Jeong, Cherlhyun; Nam, Gi-Hoon; Kwon, Minsu; Kim, In-San.
Afiliación
  • Choi Y; SHIFTBIO INC, Seoul, Republic of Korea.
  • Kim SA; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea.
  • Jung H; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea.
  • Kim E; Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
  • Kim YK; SHIFTBIO INC, Seoul, Republic of Korea.
  • Kim S; Department of Otolaryngology, University of Ulsan College of Medicine, Seoul, Republic of Korea.
  • Kim J; SHIFTBIO INC, Seoul, Republic of Korea.
  • Lee Y; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea.
  • Jo MK; Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
  • Woo J; SHIFTBIO INC, Seoul, Republic of Korea.
  • Cho Y; SHIFTBIO INC, Seoul, Republic of Korea.
  • Lee D; SHIFTBIO INC, Seoul, Republic of Korea.
  • Choi H; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea.
  • Jeong C; Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
  • Nam GH; Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul, Republic of Korea.
  • Kwon M; Research Animal Resource Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
  • Kim IS; Research Animal Resource Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
J Immunother Cancer ; 12(7)2024 Jul 15.
Article en En | MEDLINE | ID: mdl-39009452
ABSTRACT

BACKGROUND:

Triple-negative breast cancer (TNBC) poses unique challenges due to its complex nature and the need for more effective treatments. Recent studies showed encouraging outcomes from combining paclitaxel (PTX) with programmed cell death protein-1 (PD-1) blockade in treating TNBC, although the exact mechanisms behind the improved results are unclear.

METHODS:

We employed an integrated approach, analyzing spatial transcriptomics and single-cell RNA sequencing data from TNBC patients to understand why the combination of PTX and PD-1 blockade showed better response in TNBC patients. We focused on toll-like receptor 4 (TLR4), a receptor of PTX, and its role in modulating the cross-presentation signaling pathways in tumor-associated macrophages (TAMs) within the tumor microenvironment. Leveraging insights obtained from patient-derived data, we conducted in vitro experiments using immunosuppressive bone marrow-derived macrophages (iBMDMs) to validate if PTX could augment the cross-presentation and phagocytosis activities. Subsequently, we extended our study to an in vivo murine model of TNBC to ascertain the effects of PTX on the cross-presentation capabilities of TAMs and its downstream impact on CD8+ T cell-mediated immune responses.

RESULTS:

Data analysis from TNBC patients revealed that the activation of TLR4 and cross-presentation signaling pathways are crucial for the antitumor efficacy of PTX. In vitro studies showed that PTX treatment enhances the cross-presentation ability of iBMDMs. In vivo experiments demonstrated that PTX activates TLR4-dependent cross-presentation in TAMs, improving CD8+ T cell-mediated antitumor responses. The efficacy of PTX in promoting antitumor immunity was elicited when combined with PD-1 blockade, suggesting a complementary interaction.

CONCLUSIONS:

This study reveals how PTX boosts the effectiveness of PD-1 inhibitors in treating TNBC. We found that PTX activates TLR4 signaling in TAMs. This activation enhances their ability to present antigens, thereby boosting CD8+ T cell antitumor responses. These findings not only shed light on PTX's immunomodulatory role in TNBC but also underscore the potential of targeting TAMs' antigen presentation capabilities in immunotherapy approaches.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Paclitaxel / Neoplasias de la Mama Triple Negativas / Macrófagos Asociados a Tumores Idioma: En Revista: J Immunother Cancer Año: 2024 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Paclitaxel / Neoplasias de la Mama Triple Negativas / Macrófagos Asociados a Tumores Idioma: En Revista: J Immunother Cancer Año: 2024 Tipo del documento: Article