Analysis of Negative-Strand RNA Viruses by RT-qPCR: Rift Valley Fever Virus and Toscana Virus.
Methods Mol Biol
; 2824: 67-80, 2024.
Article
en En
| MEDLINE
| ID: mdl-39039406
ABSTRACT
RT-qPCR allows the detection of viruses and the monitoring of viral replication. This technique was extensively employed during the SARS-CoV-2 pandemic, where it demonstrated its efficiency and robustness. Here we describe the analysis of Rift Valley fever and Toscana virus infections over time, achieved through the RT-qPCR quantification of the viral genome. We further elaborate on the method to discriminate between genomic and antigenomic viral RNAs by using primers specific for each strand during the reverse transcription step.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Fiebre del Valle del Rift
/
Virus de la Fiebre del Valle del Rift
/
ARN Viral
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article