Robust and customizable spheroid culture system for regenerative medicine.
Biofabrication
; 16(4)2024 Aug 02.
Article
en En
| MEDLINE
| ID: mdl-39053497
ABSTRACT
Three-dimensional cell spheroids show promise for the reconstruction of native tissues. Herein, we report a sophisticated, uniform, and highly reproducible spheroid culture system for tissue reconstruction. A mesh-integrated culture system was designed to precisely control the uniformity and reproducibility of spheroid formation. Furthermore, we synthesized hexanoyl glycol chitosan, a material with ultralow cell adhesion properties, to further improve spheroid formation efficiency and biological function. Our results demonstrate improved biological function in various types of cells and ability to generate spheroids with complex structures composed of multiple cell types. In conclusion, our spheroid culture system offers a highly effective and widely applicable approach to generating customized spheroids with desired structural and biological features for a variety of biomedical applications.
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Base de datos:
MEDLINE
Asunto principal:
Esferoides Celulares
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Técnicas de Cultivo de Célula
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Medicina Regenerativa
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Quitosano
Idioma:
En
Revista:
Biofabrication
Asunto de la revista:
BIOTECNOLOGIA
Año:
2024
Tipo del documento:
Article