Transcriptional noise, gene activation, and roles of SAGA and Mediator Tail measured using nucleotide recoding single-cell RNA-seq.
Cell Rep
; 43(8): 114593, 2024 Aug 27.
Article
en En
| MEDLINE
| ID: mdl-39102335
ABSTRACT
We describe a time-resolved nascent single-cell RNA sequencing (RNA-seq) approach that measures gene-specific transcriptional noise and the fraction of active genes in S. cerevisiae. Most genes are expressed with near-constitutive behavior, while a subset of genes show high mRNA variance suggestive of transcription bursting. Transcriptional noise is highest in the cofactor/coactivator-redundant (CR) gene class (dependent on both SAGA and TFIID) and strongest in TATA-containing CR genes. Using this approach, we also find that histone gene transcription switches from a low-level, low-noise constitutive mode during M and M/G1 to an activated state in S phase that shows both an increase in the fraction of active promoters and a switch to a noisy and bursty transcription mode. Rapid depletion of cofactors SAGA and MED Tail indicates that both factors play an important role in stimulating the fraction of active promoters at CR genes, with a more modest role in transcriptional noise.
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MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Activación Transcripcional
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Proteínas de Saccharomyces cerevisiae
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Análisis de la Célula Individual
Idioma:
En
Revista:
Cell Rep
Año:
2024
Tipo del documento:
Article