The role and mechanism of ß-catenin-mediated skeletal muscle satellite cells in osteoporotic fractures by Jian-Pi-Bu-Shen formula.

ABSTRACT

Osteoporosis is a metabolic bone disease. ß-Catenin is associated with fractures. Jian-Pi-Bu-Shen (JPBS) can promote the healing of osteoporotic fractures (OPF). However, the mechanism of ß-catenin-mediated skeletal muscle satellite cells (SMSCs) in OPF by the JPBS is unclear. SMSCs were isolated and divided into five groups. The results showed that the survival rate of SMSCs was significantly higher in the low, medium, and high dose JPBS-containing serum groups after 7 days of incubation. The ALP activity and the number of SMSCs mineralized in the JPBS-containing serum intervention group were elevated. Axin, GSK-3ß, ß-catenin siRNAs were constructed and transfected into cells. Transfection of siRNAs reduced Axin, GSK-3ß, and ß-catenin expressions, respectively. ß-Catenin-siRNA reversed ALP activity, the number of SMSCs mineralized, and the expression of ß-catenin, BMP2, Runx2, COL-I, SP7/Ostrix, Osteocalcin, and BMP-7. Transcriptomic results suggested that the TNF signaling pathway associated with OPF was enriched. SD rats were subjected to the construction of OPF model by removing the ovaries. JPBS decreased the levels of PINP, ALP, CTX, and NTX through ß-catenin in OPF rats, while increasing Runx2, ß-catenin expressions through ß-catenin at the broken end of fractures. Moreover, JPBS decreased BMC, BMD, and BV/TV and improved pathological damage through ß-catenin in OPF rats. JPBS decreased the expression of Axin, GSK-3ß mRNA, and protein, but increased the expressions of ß-catenin, Pax7, COL-II, COL-II, BMP2, and Runx2 through ß-catenin in OPF rats. In conclusion, JPBS inhibits Axin/GSK-3ß expression, activates the ß-catenin signaling, and promotes the osteogenic differentiation of SMSCs.