Donor Fractions of Cell-Free DNA Are Elevated During CLAD But Not During Infectious Complications After Lung Transplantation.
Transpl Int
; 37: 12772, 2024.
Article
en En
| MEDLINE
| ID: mdl-39114640
ABSTRACT
During the last few years, cell-free DNA (cfDNA) has emerged as a possible non-invasive biomarker for prediction of complications after lung transplantation. We previously published a proof-of-concept study using a digital droplet polymerase chain reaction (ddPCR)-based method for detection of cfDNA. In the current study, we aimed to further evaluate the potential clinical usefulness of detecting chronic lung allograft dysfunction (CLAD) using three different ddPCR applications measuring and calculating the donor fraction (DF) of cfDNA as well as one method using the absolute amount of donor-derived cfDNA. We analyzed 246 serum samples collected from 26 lung transplant recipients. Nine of the patients had ongoing CLAD at some point during follow-up. All four methods showed statistically significant elevation of the measured variable in the CLAD samples compared to the non-CLAD samples. The results support the use of ddPCR-detected cfDNA as a potential biomarker for prediction of CLAD. These findings need to be validated in a subsequent prospective study.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Biomarcadores
/
Trasplante de Pulmón
/
Ácidos Nucleicos Libres de Células
Idioma:
En
Revista:
Transpl Int
/
Transplant international
/
Transplant. int
Asunto de la revista:
TRANSPLANTE
Año:
2024
Tipo del documento:
Article