Conformational change-based fluorometric aptasensor for sensitive cadmium(II) detection in fruits and vegetables.
Anal Methods
; 16(34): 5826-5834, 2024 Aug 29.
Article
en En
| MEDLINE
| ID: mdl-39143932
ABSTRACT
Cadmium (Cd2+) is a highly toxic heavy metal that can accumulate in the human body through contaminated food and water, posing great health risks. In this study, a label-free fluorescent aptasensor based on SYBR Green I (SGI) for the rapid and sensitive detection of Cd2+ in food samples was designed. The aptasensor utilizes a Cd2+-specific aptamer (Cd-(21)) and its complementary strand (CSCd-(21)) to form a double-stranded DNA (dsDNA) structure in the absence of Cd2+. SGI intercalates into the dsDNA, resulting in a strong fluorescence signal. In the presence of Cd2+, the aptamer undergoes a conformational change, preventing the formation of dsDNA and leading to a decrease in fluorescence intensity. Under optimized conditions, the aptasensor exhibited a linear response to Cd2+ concentrations ranging from 0.11 to 157.37 ng mL-1, with a limit of detection (LOD) of 0.07 ng mL-1. The aptasensor demonstrated high specificity and was successfully applied to detect Cd2+ in fruits and vegetables, with satisfactory recovery rates (95-111%). The proposed aptasensor provides a promising tool for the rapid and sensitive detection of Cd2+ in food.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Verduras
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Cadmio
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Técnicas Biosensibles
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Aptámeros de Nucleótidos
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Límite de Detección
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Frutas
Idioma:
En
Revista:
Anal Methods
Año:
2024
Tipo del documento:
Article