Chemical Composition of Five Lamiaceae Essential Oils and Their Insecticidal and Phytotoxic Activity.

ABSTRACT

The Lamiaceae family is widely distributed worldwide. In this study, we investigated the insecticidal activity of five Lamiaceae essential oils against Thrips flavus Schrank and the phytotoxic activity against Glycine max (L.) Merr., Zea mays L., Portulaca oleracea L., and Echinochloa oryzoides (Ard.) Fritsch. Then, the chemical composition of the five essential oils was analyzed by using gas chromatography-mass spectrometry (GC-MS). The five Lamiaceae essential oils were melissa, basil, rosemary, negundo chastetree, and salvia. The main constituents of the five Lamiaceae essential oils were preliminarily determined to be as follows α-pinene and 1,8-cineole in the rosemary essential oil; ß-pinene, γ-terpinene, and d-limonene in the negundo chastetree essential oil; ß-cadinene and isolongifolen-5-one in the melissa essential oil; 5-allylguaiacol in the basil essential oil; and isopropyl myristate, linalyl acetate, and linalool in the salvia essential oil. Using a bioassay, it was found that, among the five essential oils, the melissa essential oil exhibited the lowest LC50 value, which was 0.18 mg/mL, and the salvia essential oil exhibited the highest LC50 value, which was 0.42 mg/mL. The control efficacy of the five essential oils significantly increased with time and concentration in pot experiments. The negundo chastetree, basil, rosemary, and salvia essential oils at 900.00 g a.i.·hm-2 showed high control efficacy against T. flavus, with values higher than 90%. Female thrips were attracted to the negundo chastetree essential oil. The five essential oils were also tested for their effects on the germination rate, germination potential, germination index, and shoot length of G. max, Z. mays, P. oleracea, and E. oryzoides. The basil essential oil significantly inhibited the germination of P. oleracea, with germination at a concentration of 1.0 mg/mL being only 11.11 ± 5.09%. This study provides a reference for the development of botanical pesticides to control T. flavus, crops, and weeds.