SARS-CoV-2 spike aggravates lupus nephritis and lung fibrosis in systemic lupus erythematosus.
Lupus Sci Med
; 11(2)2024 Sep 30.
Article
en En
| MEDLINE
| ID: mdl-39349051
ABSTRACT
OBJECTIVE:
COVID-19 induces the development of autoimmune diseases, including SLE, which are characterised by inflammation, autoantibodies and thrombosis. However, the effects of COVID-19 on SLE remain unclear.METHODS:
We investigated the effects of COVID-19 on SLE development and progression in three animal models. Plasmids encoding SARS-CoV-2 spike protein and ACE2 receptor were injected into R848-induced BALB/C lupus mice, R848-induced IL-1 receptor antagonist knockout (KO) lupus mice and MRL/lpr mice. Serum levels of albumin and autoantibodies, lymphocyte phenotypes and tissue histology were evaluated.RESULTS:
In R848-induced BALB/C lupus mice, the SARS-CoV-2 spike protein increased autoantibody and albumin levels compared with vehicle and mock treatments. These mice also exhibited splenomegaly, which was further exacerbated by the spike protein. Flow cytometric analysis revealed elevated T helper 1 cell counts, and histological analysis indicated increased levels of the fibrosis marker protein α-smooth muscle actin. In KO mice, the spike protein induced splenomegaly, severe kidney damage and pronounced lung fibrosis. In the MRL/lpr group, spike protein increased the serum levels of autoantibodies, albumin and the thrombosis marker chemokine (C-X-C motif) ligand 4.CONCLUSION:
COVID-19 accelerated the development and progression of lupus by inducing autoantibody production, fibrosis and thrombosis.Palabras clave
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Base de datos:
MEDLINE
Asunto principal:
Autoanticuerpos
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Nefritis Lúpica
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Ratones Noqueados
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Modelos Animales de Enfermedad
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Glicoproteína de la Espiga del Coronavirus
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SARS-CoV-2
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COVID-19
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Lupus Eritematoso Sistémico
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Ratones Endogámicos BALB C
Idioma:
En
Revista:
Lupus Sci Med
Año:
2024
Tipo del documento:
Article