Protein conformation and reversed-phase high-performance liquid chromatography.
J Chromatogr
; 317: 93-101, 1984 Dec 28.
Article
en En
| MEDLINE
| ID: mdl-6530457
ABSTRACT
The structure of a series of proteins has been investigated by circular dichroism, fluorescence and visible spectroscopy as well as by differential scanning calorimetry under reversed-phase high-performance liquid chromatography elution conditions. These studies show that 1-propanol, a typical eluent, induces a reversible conformational change in proteins to an apparently ordered, helical form. This structural transition occurs in the range of propanol concentrations that produces elution of a particular protein. The possible relationship between this conformational change and protein elution is considered.
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Base de datos:
MEDLINE
Asunto principal:
Conformación Proteica
/
Cromatografía Líquida de Alta Presión
Idioma:
En
Revista:
J Chromatogr
Año:
1984
Tipo del documento:
Article