Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk.
Proc Natl Acad Sci U S A
; 92(6): 1866-70, 1995 Mar 14.
Article
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| MEDLINE
| ID: mdl-7534404
ABSTRACT
Htk is a receptor protein-tyrosine kinase that is related to the EPH subfamily of tyrosine kinases. The receptor has a wide tissue distribution including expression in several myeloid hematopoietic cell lines. Using an Htk-Fc fusion protein, a protein ligand for this receptor was expression cloned from the murine kidney mesangial cell line SV40MES 13. The Htk ligand cDNA encodes a transmembrane protein of 336 amino acids. Binding competition experiments demonstrated a Kd of 535 pM for binding of Htk-Fc to the Htk ligand. Incubation of 3T3 cells expressing Htk with COS-7 cells expressing the ligand resulted in tyrosine phosphorylation of Htk. The ligand, like its receptor, is widely expressed and may function in a variety of tissues. However, we localized hematopoietic expression of Htk to the monocytic lineage, suggesting that the ligand may play a role in differentiation and/or proliferation of these cells.
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1
Base de datos:
MEDLINE
Asunto principal:
Proteínas Tirosina Quinasas Receptoras
/
Proteínas de la Membrana
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
1995
Tipo del documento:
Article