The expression of carbohydrate blood group antigens correlates with heat resistance.
J Cell Sci
; 108 ( Pt 4): 1691-701, 1995 Apr.
Article
en En
| MEDLINE
| ID: mdl-7615686
ABSTRACT
Recent data indicate that cells may resist heat shock via more than one route heat shock protein synthesis and other still ill-defined mechanisms. We investigated this phenomenon using four types of cells derived from a single rat colon carcinoma clones REGb and PROb; PRO A+, a glycosylation variant of PROb selected for its high expression of blood group A antigen; and Ph8, a thermoresistant variant of PROb selected by repeated sublethal heat treatments. Basal heat resistance was clearly associated with the level of cell surface expression of blood group H and A antigens. Biosynthesis of these carbohydrate structures requires two glycosyltransferases, H and A enzymes, whose activities are also correlated with basal heat resistance. In addition, heat sensitive REGb cells were rendered more resistant by transfection with the gene encoding for H enzyme, allowing expression of H antigen. Thus, these terminal glycosylations could play a role as cellular protectors against heat treatment. Blood group carbohydrate antigens were mainly located on O-linked carbohydrate chains of a major glycoprotein of 200 kDa and to a lesser extent on N-linked chains. Only trace amounts were present as glycolipids.
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Base de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo ABO
/
Expresión Génica
Idioma:
En
Revista:
J Cell Sci
Año:
1995
Tipo del documento:
Article