High-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres.
J Chromatogr B Biomed Sci Appl
; 688(1): 47-55, 1997 Jan 10.
Article
en En
| MEDLINE
| ID: mdl-9029312
ABSTRACT
The combined solid-phase extraction (Isolute PRS columns) and reversed-phase gradient HPLC method presented provides a sensitive, reproducible and selective quantification of carnosine, balenine, homocarnosine, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres. Recoveries were 91-115%. Lower limits of detection were 0.005-0.010 mmol kg-1 dry muscle. The compounds were isolated from other physiological amino acids and small peptides and resolved within a single chromatographic run of 55 min. Concentrations of these compounds in equine myocardium, diaphragm, skeletal muscle, camel muscle and individual muscle fibres of both species are presented for the first time.
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Base de datos:
MEDLINE
Asunto principal:
Camelus
/
Cromatografía Líquida de Alta Presión
/
Dipéptidos
/
Histidina
/
Caballos
/
Imidazoles
/
Músculos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Asunto de la revista:
QUIMICA CLINICA
Año:
1997
Tipo del documento:
Article