Determination of amiprilose in human plasma by high-performance liquid chromatography with fluorimetric detection.
J Chromatogr B Biomed Sci Appl
; 692(1): 149-56, 1997 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-9187394
ABSTRACT
A reversed-phase HPLC method to quantify amiprilose in human plasma is described. The method involves liquid-liquid extraction of amiprilose and the internal standard from plasma. The extracted compounds are derivatized with 1,8-naphthalic dicarboxylic acid using 2-chloro-1-methylpyridinium iodide as a coupling reagent. The derivatized products are separated on a reversed-phase column and monitored fluorimetrically using 280 nm and 340 nm as excitation and emission wavelengths, respectively. The derivatized products which exhibit two peaks on chromatogram, are shown to be the interconvertible isomers. This assay has been used in pharmacokinetic studies of amiprilose in humans.
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Base de datos:
MEDLINE
Asunto principal:
Antivirales
/
Adyuvantes Inmunológicos
/
Glucosamina
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Asunto de la revista:
QUIMICA CLINICA
Año:
1997
Tipo del documento:
Article