Replication-competent retroviral vectors for expressing genes in avian cells in vitro and in vivo.
Mol Biotechnol
; 7(3): 289-98, 1997 Jun.
Article
en En
| MEDLINE
| ID: mdl-9219242
ABSTRACT
Replication-competent retroviral vectors based on Rous sarcoma virus (RSV) are becoming increasingly popular for expressing genes in both primary cell cultures and embryonic chick tissues in ovo. In this article, we review the features of RSV and its life cycle that make it suitable for use as a vector. We describe the design and use of the RCAS and RCAS (BP) series of vectors, which are currently the most widely used RSV-based vectors, illustrating both their strengths and weakness. Finally, we outline laboratory protocols suitable for the banding of these retroviral vectors.
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Base de datos:
MEDLINE
Asunto principal:
Replicación Viral
/
Expresión Génica
/
Virus del Sarcoma Aviar
/
Vectores Genéticos
Idioma:
En
Revista:
Mol Biotechnol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Año:
1997
Tipo del documento:
Article