The significance of Ras guanine nucleotide exchange factor, son of sevenless protein, in renal cell carcinoma cell lines.
J Urol
; 158(3 Pt 1): 908-11, 1997 Sep.
Article
en En
| MEDLINE
| ID: mdl-9258117
ABSTRACT
PURPOSE:
The aim of the present study is to clarify the significance of the Ras guanine-nucleotide exchange reaction in the proliferation of human renal cell carcinoma cell lines. MATERIALS ANDMETHODS:
We examined the expression of human son of sevenless-1 (hSos-1) protein and the epidermal growth factor (EGF) receptor in human renal cell carcinoma cell lines by Western blot analysis. Additionally, a dominant negative H-ras mutant, N116Y, which is known to inhibit the Ras guanine-nucleotide exchange reaction, was transfected into these cell lines by lipofection.RESULTS:
Human renal cell carcinoma cell lines expressed much higher amounts of the EGF receptor and hSos-1 protein than normal kidney tissue. Moreover, the N116Y ras mutant could strongly suppress cellular proliferation in these cell lines.CONCLUSIONS:
Augmentation of the Ras guanine-nucleotide exchange reaction might be essential to the proliferation of human renal cell carcinoma cells.
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Base de datos:
MEDLINE
Asunto principal:
Carcinoma de Células Renales
/
Proteínas
/
Factor 2 Eucariótico de Iniciación
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Receptores ErbB
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Neoplasias Renales
Idioma:
En
Revista:
J Urol
Año:
1997
Tipo del documento:
Article