Involvement of integrins and the cytoskeleton in modulation of skeletal muscle glycogen synthesis by changes in cell volume.
FEBS Lett
; 417(1): 101-3, 1997 Nov 03.
Article
en En
| MEDLINE
| ID: mdl-9395084
ABSTRACT
Muscle glycogen synthesis is modulated by physiologically relevant changes in cell volume. We have investigated the possible involvement of integrin-extracellular matrix interactions in this process using primary cultures of rat skeletal muscle subject to hypo- or hyper-osmotic exposure with integrin binding peptide GRGDTP to disrupt integrin actions and the inactive analogue GRGESP as control. Osmotically induced increases (77%) and decreases (34%) in glycogen synthesis (D-[14C]glucose incorporation into glycogen) were prevented by GRGDTP (but not GRGESP) without affecting glucose transport. Cytoskeletal disruption with cytochalasin D or colchicine had similar effects to GRGDTP. Osmotically induced modulation of muscle glycogen synthesis involves integrin-extracellular matrix interactions and cytoskeletal elements, possibly as components of a cell-volume 'sensing' mechanism.
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Base de datos:
MEDLINE
Asunto principal:
Citoesqueleto
/
Integrinas
/
Músculo Esquelético
/
Glucógeno
Idioma:
En
Revista:
FEBS Lett
Año:
1997
Tipo del documento:
Article