Molecular cloning of bovine thyrotropin-releasing hormone receptor gene.
J Vet Med Sci
; 60(1): 123-7, 1998 Jan.
Article
en En
| MEDLINE
| ID: mdl-9492373
ABSTRACT
The genomic DNA encoding bovine Thyrotropin-Releasing Hormone Receptor (TRHR) was isolated from a bovine (Holstein) genomic library. Using PCR fragments of bovine candidate TRHR Transmembrane domain (TMD-III-IV) and C-terminus domain of mouse TRHR cDNA as probes, 9 x 10(5) plaques were screened to obtain several clones each containing the N-terminus or C-terminus domain. The bovine TRHR gene encoded 398 amino acids and has a long intron. The identity of the deduced amino acid sequence of bovine TRHR exceeded 88% that of mouse, rat or human. RT-PCR analysis indicated TRHR mRNA to be expressed in the pituitary and brain.
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Base de datos:
MEDLINE
Asunto principal:
Receptores de Hormona Liberadora de Tirotropina
Idioma:
En
Revista:
J Vet Med Sci
Asunto de la revista:
MEDICINA VETERINARIA
Año:
1998
Tipo del documento:
Article