A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethybenzene, and xylene (BTEX) sensing.
Appl Environ Microbiol
; 64(7): 2730-5, 1998 Jul.
Article
en En
| MEDLINE
| ID: mdl-9647859
ABSTRACT
A tod-luxCDABE fusion was constructed and introduced into the chromosome of Pseudomonas putida F1, yielding the strain TVA8. This strain was used to examine the induction of the tod operon when exposed to benzene, toluene, ethylbenzene, and xylene (BTEX) compounds and aqueous solutions of JP-4 jet fuel constituents. Since this system contained the complete lux cassette (luxCDABE), bacterial bioluminescence in response to putative chemical inducers of the tod operon was measured on-line in whole cells without added aldehyde substrate. There was an increasing response to toluene concentrations from 30 micrograms/liter to 50 mg/liter, which began to saturate at higher concentrations. The detection limit was 30 micrograms/liter. There was a significant light response to benzene, m- and p-xylenes, phenol, and water-soluble JP-4 jet fuel components, but there was no bioluminescence response upon exposure to o-xylene. The transposon insertion was stable and had no negative effect on cell growth.
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Base de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
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Regulación Bacteriana de la Expresión Génica
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Pseudomonas putida
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Hidrocarburos Aromáticos
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
1998
Tipo del documento:
Article