RESUMEN
Barrier membranes for guided bone regeneration (GBR) were prepared by a solvent casting method using solutions of poly(L-lactic acid) (PLLA) and chitosan. PLLA and PLLA/chitosan membranes were treated with ammonia gas plasma. PLLA/chitosan membranes were successfully fabricated, and the surface of the PLLA/chitosan membrane was clearly modified by NH3 plasma treatment according to attenuated total reflectance (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) analyses. Additionally, water contact angle testing indicated that the hydrophilicity of these membranes was significantly increased. MG-63 cells were cultured on each type of membrane, and cell viability was examined using an MTT assay. After one week of culturing, MG-63 cells were more abundant on PLLA/chitosan membranes than on PLLA membranes. The cell viability of PLLA/chitosan membranes with plasma treatment was significantly higher than that of PLLA membranes. These results suggest that this plasma-treated membrane is suitable for GBR and is a promising source of bioactive membrane material for bone regeneration.
Asunto(s)
Amoníaco/química , Regeneración Ósea , Adhesión Celular , Regeneración Tisular Dirigida/instrumentación , Ácido Láctico/química , Membranas Artificiales , Osteoblastos/citología , Polímeros/química , Línea Celular , Humanos , Microscopía Electrónica de Rastreo , Espectroscopía de Fotoelectrones , Poliésteres , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Highly ordered nanotube oxide layers were developed on low rigidity quaternary beta-type Ti-35Nb-5Ta-7Zr alloy by controlled anodic oxidation in electrolyte containing 1 M H3PO4 and 0.5 wt% NaF at room temperature. The diameters of the nanotubes formed were in the range of 30 to 80 nm. Electrochemical corrosion behavior of the nanotubular alloy was studied in Ringer's solution at 37 +/- 1 degrees C using potentiodynamic polarization and AC Impedance. The result of the study showed that nanotube formation on the surface affect the passivation behavior of the quaternary alloy significantly. However the corrosion current density was considerably higher for the nanotubular alloy.