RESUMEN
Barrier membranes for guided bone regeneration (GBR) were prepared by a solvent casting method using solutions of poly(L-lactic acid) (PLLA) and chitosan. PLLA and PLLA/chitosan membranes were treated with ammonia gas plasma. PLLA/chitosan membranes were successfully fabricated, and the surface of the PLLA/chitosan membrane was clearly modified by NH3 plasma treatment according to attenuated total reflectance (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) analyses. Additionally, water contact angle testing indicated that the hydrophilicity of these membranes was significantly increased. MG-63 cells were cultured on each type of membrane, and cell viability was examined using an MTT assay. After one week of culturing, MG-63 cells were more abundant on PLLA/chitosan membranes than on PLLA membranes. The cell viability of PLLA/chitosan membranes with plasma treatment was significantly higher than that of PLLA membranes. These results suggest that this plasma-treated membrane is suitable for GBR and is a promising source of bioactive membrane material for bone regeneration.
Asunto(s)
Amoníaco/química , Regeneración Ósea , Adhesión Celular , Regeneración Tisular Dirigida/instrumentación , Ácido Láctico/química , Membranas Artificiales , Osteoblastos/citología , Polímeros/química , Línea Celular , Humanos , Microscopía Electrónica de Rastreo , Espectroscopía de Fotoelectrones , Poliésteres , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
We developed a novel bone grafting material that incorporates autogenous teeth (AutoBT), and provided the basis for its clinical application. AutoBT contains organic and inorganic mineral components and is prepared from autogenous grafting material, thus eliminating the risk of an immune reaction that may lead to rejection. AutoBT was used at the time of implant placement, simultaneously with osteoinduction surgery, and excellent bony healing by osteoinduction and osteoconduction was confirmed.