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1.
Biomacromolecules ; 25(6): 3784-3794, 2024 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-38743836

RESUMEN

The effective regeneration of large bone defects via bone tissue engineering is challenging due to the difficulty in creating an osteogenic microenvironment. Inspired by the fibrillar architecture of the natural extracellular matrix, we developed a nanoscale bioengineering strategy to produce bone fibril-like composite scaffolds with enhanced osteogenic capability. To activate the surface for biofunctionalization, self-adaptive ridge-like nanolamellae were constructed on poly(ε-caprolactone) (PCL) electrospinning scaffolds via surface-directed epitaxial crystallization. This unique nanotopography with a markedly increased specific surface area offered abundant nucleation sites for Ca2+ recruitment, leading to a 5-fold greater deposition weight of hydroxyapatite than that of the pristine PCL scaffold under stimulated physiological conditions. Bone marrow mesenchymal stem cells (BMSCs) cultured on bone fibril-like scaffolds exhibited enhanced adhesion, proliferation, and osteogenic differentiation in vitro. In a rat calvarial defect model, the bone fibril-like scaffold significantly accelerated bone regeneration, as evidenced by micro-CT, histological histological and immunofluorescence staining. This work provides the way for recapitulating the osteogenic microenvironment in tissue-engineered scaffolds for bone repair.


Asunto(s)
Regeneración Ósea , Células Madre Mesenquimatosas , Osteogénesis , Poliésteres , Ingeniería de Tejidos , Andamios del Tejido , Animales , Andamios del Tejido/química , Ratas , Regeneración Ósea/efectos de los fármacos , Células Madre Mesenquimatosas/citología , Osteogénesis/efectos de los fármacos , Osteogénesis/fisiología , Ingeniería de Tejidos/métodos , Poliésteres/química , Diferenciación Celular , Ratas Sprague-Dawley , Materiales Biomiméticos/química , Materiales Biomiméticos/farmacología , Células Cultivadas , Proliferación Celular , Cráneo/lesiones , Cráneo/patología , Durapatita/química , Durapatita/farmacología
2.
ACS Appl Mater Interfaces ; 16(15): 18658-18670, 2024 Apr 17.
Artículo en Inglés | MEDLINE | ID: mdl-38587811

RESUMEN

Three-dimensional (3D)-printed biodegradable polymer scaffolds are at the forefront of personalized constructs for bone tissue engineering. However, it remains challenging to create a biological microenvironment for bone growth. Herein, we developed a novel yet feasible approach to facilitate biomimetic mineralization via self-adaptive nanotopography, which overcomes difficulties in the surface biofunctionalization of 3D-printed polycaprolactone (PCL) scaffolds. The building blocks of self-adaptive nanotopography were PCL lamellae that formed on the 3D-printed PCL scaffold via surface-directed epitaxial crystallization and acted as a linker to nucleate and generate hydroxyapatite crystals. Accordingly, a uniform and robust mineralized layer was immobilized throughout the scaffolds, which strongly bound to the strands and had no effect on the mechanical properties of the scaffolds. In vitro cell culture experiments revealed that the resulting scaffold was biocompatible and enhanced the proliferation and osteogenic differentiation of mouse embryolous osteoblast cells. Furthermore, we demonstrated that the resulting scaffold showed a strong capability to accelerate in vivo bone regeneration using a rabbit bone defect model. This study provides valuable opportunities to enhance the application of 3D-printed scaffolds in bone repair, paving the way for translation to other orthopedic implants.


Asunto(s)
Osteogénesis , Andamios del Tejido , Ratones , Animales , Conejos , Andamios del Tejido/química , Biomimética , Regeneración Ósea , Poliésteres/química , Ingeniería de Tejidos , Impresión Tridimensional
3.
Materials (Basel) ; 14(22)2021 Nov 17.
Artículo en Inglés | MEDLINE | ID: mdl-34832341

RESUMEN

Polyacrylics have been considered for a broad range of material applications, including coatings, dental applications, and adhesives. In this experimental study, the casting potential of a group of (co)monomers belonging to the acrylic family has been explored to enable a more sustainable use of these polymer materials in the medical and veterinary science field. The individual contributions of each comonomer have been analyzed, the reaction conversion has been studied via gas chromatography (GC), the rheological behavior has been characterized via stress-controlled measurements, and the final mechanical properties have been obtained from tensile, flexure, and impact tests. The GC results allow assessing the pot life and thus the working window of the casting process. For the rheological measurements, which start from low-viscous mixtures, a novel protocol has been introduced to obtain accurate absolute data. The rheological data reflect the time dependencies of the GC data but facilitate a more direct link with the macroscopic material data. Specifically, the steep increase in the viscosity with increasing reaction time for the methyl methacrylate (MMA)/ethylene glycol dimethyl methacrylate (EGDMA) case (2% crosslinker) allows maximizing several mechanical properties: the tensile/flexure modulus, the tensile/flexure stress at break, and the impact strength. This opens the pathway to more dedicated chemistry design for corrosion casting and polyacrylic material design in general.

4.
Acta Biomater ; 90: 49-59, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-30930306

RESUMEN

Nerve conduits provide an advanced tool for repairing the injured peripheral nerve that often causes disability and mortality. Currently, the efficiency of conduits in repairing peripheral nerve is unsatisfying. Here, we show a functional nanoparticle-enhanced nerve conduit for promoting the regeneration of peripheral nerves. This conduit, which consists of gelatin-methacryloyl (GelMA) hydrogels with drug loaded poly(ethylene glycol)- poly(3-caprolactone) (MPEG-PCL) nanoparticles dispersed in the hydrogel matrix, is rapidly fabricated by a continuous three-dimensional (3D) printing process. While the 3D-printed hydrogel conduit with customized size, shape and structure provides a physical microenvironment for axonal elongation, the nanoparticles sustained release the drug to facilitate the nerve regeneration. The drug, 4-((5,10-dimethyl-6-oxo-6,10-dihydro-5H-pyrimido[5,4-b]thieno[3,2-e][1,4]diazepin-2-yl)amino) benzenesulfonamide, is a Hippo pathway inhibitor with multiple functions including improving the proliferation and migration of Schwann cells and up-regulating neurotrophic factors genes. The descried functional nerve conduit efficiently induced the recovery of sciatic injuries in morphology, histopathology and functions in vivo, showing the potential clinical application in peripheral nerve repair. STATEMENTS OF SIGNIFICANCE: Functional nerve conduit provides a promising strategy alternative to autografts. In this work, we rapidly customized a nanoparticle-enhanced conduit by the continuous bioprinting process. This nanoparticle in the conduit can release a Hippo pathway inhibitor to facilitate the nerve regeneration and function restoration. The efficacy of the conduits is comparable to that of autograft, suggesting the potential clinical applications.


Asunto(s)
Bioimpresión , Nanopartículas/química , Regeneración Nerviosa , Células de Schwann/metabolismo , Nervio Ciático/fisiología , Andamios del Tejido/química , Animales , Línea Celular , Gelatina/química , Gelatina/farmacología , Células Endoteliales de la Vena Umbilical Humana , Humanos , Masculino , Poliésteres/química , Poliésteres/farmacología , Polietilenglicoles/química , Polietilenglicoles/farmacología , Impresión Tridimensional , Ratas , Ratas Sprague-Dawley
5.
Waste Manag ; 82: 198-206, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-30509582

RESUMEN

Flame retardants are used in a wide range of plastics to extend the time-of-escape from fires. By definition, they are designed to perform this task only in case of a fire, which is then automatically the end of the plastic's lifetime. However, not all flame retardant plastic products are eventually set on fire, which is why they are abundant in plastic waste, potentially interfering with the mechanical recycling systems in place. To date, there has been little information on the influence of flame retardant additives during the mechanical recycling of solid (thermo)plastic waste. This contribution provides a comprehensive overview of the state of the art concerning the mechanical recycling of flame retardants containing polymers and plastics. In a first part, this review discusses the effect of mechanical melt reprocessing on the flame retardant properties of different recycled thermoplastic polymers, addressing questions whether the flame retardant additives are still present and effective after recycling and whether they interfere with the mechanical recycling itself. Special attention is paid to Waste from Electrical and Electronic Equipment containing flame retardants. A second part of the review lists several upgrading strategies for common polymeric waste streams that consist of adding virgin flame retardants to recycled plastics with the purpose of bringing an additional value to the compound.


Asunto(s)
Residuos Electrónicos , Retardadores de Llama , Plásticos , Polímeros , Reciclaje , Residuos Sólidos
6.
Biofabrication ; 2(1): 014107, 2010 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-20811122

RESUMEN

3D plotting by micro-extrusion is a promising layer-wise fabrication method for the production of scaffolds in thermoplastic polymers. It is a solvent-free direct technique which permits extensive control over geometry and porosity. This paper highlights the complications that arise when using this technique for the processing of thermally sensitive polymers. It has been noted that the material is subject to extensive thermal load during processing, which may result in degradation by chain scission. This negatively affects scaffold (mechanical) properties as well as predictability and repeatability of the fabrication technique. A rationale is offered as to the main causes of this thermally induced degradation during processing and tentative ideas towards a solution are equally put forward.


Asunto(s)
Biotecnología/instrumentación , Diseño Asistido por Computadora/instrumentación , Plásticos/química , Ingeniería de Tejidos/instrumentación , Andamios del Tejido , Diseño de Equipo , Análisis de Falla de Equipo , Calor , Reproducibilidad de los Resultados
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