LncRNA H19 and Target Gene-mediated Cleft Palate Induced by TCDD.

This study investigated the role of long non-coding RNAs (lncRNAs) in the development of the palatal tissues. Cleft palates in mice were induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Expression levels of long non-coding RNA H19 (lncRNA H19) and insulin-like growth factor 2 (IGF2) gene were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The rate of occurrence of cleft palate was found to be 100% by TCDD exposure, and TCDD could cause short upper limb, cerebral fissure, webbed neck, and short neck. The expression levels of lncRNA H19 and IGF2 gene specifically showed embryo age-related differences on E13, E14, and E15 in the palatal tissues. The expression levels of lncRNA H19 and IGF2 gene showed an inverse relationship on E13, E14, and E15. These findings demonstrated that lncRNA H19 and IGF2 can mediate the development of mouse cleft palate.

[Minimal invasive microscopic tooth preparation based on endodontic, periodontal and functional health].

Tooth preparation is the primary and core operation technique for dental esthetic restoration treatment, due to its effect of providing restoration space, bonding interfaces and marginal lines for dental rehabilitation after tooth tissue reduction. The concept of microscopic minimal invasive dentistry put forward the issue of conducting high-quality tooth preparation, conserve tooth-structure, protect vital pulp and periodontal tissue simultaneously. This study reviewed the concepts, physiology background, design and minimal invasive microscopic tooth preparation, and in the meantime, individualized strategies and the two core elements of tooth preparation (quantity and shape) are listed.

Micromotions and combined damages at the dental implant/bone interface.

Micromotion and fretting damages at the dental implant/bone interface are neglected for the limitation of check methods, but it is particularly important for the initial success of osseointegration and the life time of dental implant. This review article describes the scientific documentation of micromotion and fretting damages on the dental implant/bone interface. The fretting amplitude is less than 30 µm in vitro and the damage in the interface is acceptable. While in vivo, the micromotion's effect is the combination of damage in tissue level and the real biological reaction.

[Effects of different concentrations of ethylenediamine tetraacetic acid paste on removing root canal smear].

OBJECTIVE: To evaluate the effect of different concentrations of ethylenediamine tetraacetic acid (EDTA) paste on the removing root canal smear layers and the degrees of erosion on the surface of the root canal walls at the different portions of canal. METHODS: Sixty human teeth with single root were instrumented using step-back technique, then were divided into six groups and treated with different concentrations EDTA paste and NaCLO solution. Group A: 0.9% saline; group B: 5.25% NaC10+5% EDTA; group C: 5.25% NaC10+10% EDTA; group D: 5.25% NaC10+15% EDTA; group E: 5.25% NaC10+17% EDTA; group F: 5.25% NaClO+20% EDTA. Then the teeth were split, the root canals with different treatments were examined with scanning electron microscope at the coronal, middle and apical thirds for smear layers removal and the degrees of erosion. RESULTS: The effect of EDTA paste on removing smear layers at the coronal, middle thirds of the root canal increased with the concentrations. 15% EDTA paste could remove the smear layers at the coronal and middle thirds of the root canal, but the effect on apical third was invalid, and no erosion could be found in the root canal wall. 17% and 20% EDTA paste produced the erosion to the root canal wall. CONCLUSION: The concentrations of the EDTA paste can influence the effect of the removing smear layers, and the concentration beyond 17% would produce the erosion to root canal wall.

[The disinfection efficiency observation of a compound disinfectant spray on dental impression and plaster].

OBJECTIVE: The disinfection efficiency of a compound disinfectant spray with trichioro hydroxyl diphenyl ether on dental impression and plaster model, which have been contaminated by pathogens, were evaluated in this study. METHODS: As experimental group, germ-free alginate impressions and plaster models were sprayed with the compound disinfectant of different density trichloro hydroxyl diphenyl ether or indophors for 5, 10 and is mm, after which were smeared with five tested pathogens, including Staphylococcus acre us, Escherichia cali, Saccharomyces albicans, Streptococcus mutans and black spore variants of Bacillus subtilis. The colonies were counted after sampling, inoculate and culture, which were used to deduce the killing logarithm value as the standard of the disinfecting efficiency. RESULTS: the compound disinfectant spray with 3000 mg x L(-1) triebloro hydroxyl diphenyl ether was effective to all tested pathogens for 10 mm whatever on the impressions or the plaster models. The disinfectant spray with tame concentration was more effective on the alginate impression than on the plaster model in the same time (P = 0.000). CONCLUSION: The compound disinfectant spray with trichioro hydroxyl diphenyl ether is an effective antiseptics for alginate impressions and plaster models.