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1.
Macromol Rapid Commun ; 45(2): e2300467, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-37863475

RESUMEN

Conductive polymer hydrogels are vital in strain sensors, yet achieving high resilience and toughness is a challenge. This study employs a prestretch method to engineer a tough conductive polymer hydrogel with sufficient resilience. Initially, a blend film of polyvinylalcohol (PVA) and ethylene-vinyl alcohol copolymer (EVOH) is prepared through solution casting, followed by a swelling process to form a PVA-EVOH hydrogel. This hydrogel, with PVA crystallites as crosslinking points, exhibits high toughness. The hydrogel is then immersed in pyrrole and ferric chloride solutions for in-situ polymerization of polypyrrole (PPy), creating a conductive PPy/PVA-EVOH hydrogel. Finally, a 200% prestretch is applied, breaking short chains within the network, eliminating energy dissipation at low strains. This results in a hydrogel with a 100% elastic deformation range, while maintaining high fracture toughness (1700 J m-2 ). The prestretched PPy/PVA-EVOH hydrogel functions as a strain sensor with low hysteresis, providing consistent strain measurements during loading and unloading. This outperforms the non-prestretched sample, which shows inconsistent responses between stretching and releasing.


Asunto(s)
Polímeros , Resiliencia Psicológica , Hidrogeles , Pirroles , Alcohol Polivinílico , Conductividad Eléctrica
2.
Int J Mol Sci ; 23(7)2022 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-35409274

RESUMEN

Malignant mesothelioma (MM) is a lethal tumor originating in the mesothelium with high chemotherapeutic resistance. Cancer stem cells (CSCs) persist in tumors and are critical targets responsible for tumor resistance and recurrence. The identification and characterization of CSCs may help develop effective treatment for MM. The objective of this study was to evaluate the therapeutic effect of molecular targeted radiotherapy by 177Lu-labeled immunoliposomes (177Lu-ILs) on CSCs of mesothelioma. MM CSCs were sorted based on CD26/CD24 expression level and their functional significances were established by small interference RNA. CSC potential of MM was evaluated for drug resistance, cell invasion, and cell growth rate in vitro. CSC metabolism was evaluated with the uptake of 18F-FDG. Therapeutic effects of 177Lu-labeled immunoliposomes targeting CD26 and CD24 were evaluated in vitro through proliferation and apoptotic assays. CSCs sorted from H28 cells exhibited significant drug resistance and enhanced proliferative activity as well as increased metabolism indicated by higher 18F-FDG uptake. Treatment with 177Lu-ILs, compared with 177Lu-CL and ILs, showed enhanced therapeutic effects on inhibition of proliferation, up-regulation of apoptosis, and suppression of CD26 and CD24 expression. Thus, our results suggest that molecular radiotherapy targeting both CD26 and CD24 could be a promising approach for CSC-targeting therapy for MM.


Asunto(s)
Mesotelioma Maligno , Mesotelioma , Línea Celular Tumoral , Dipeptidil Peptidasa 4/metabolismo , Fluorodesoxiglucosa F18/metabolismo , Humanos , Liposomas/metabolismo , Mesotelioma/tratamiento farmacológico , Mesotelioma/genética , Mesotelioma/radioterapia , Células Madre Neoplásicas/metabolismo
3.
J Cell Mol Med ; 25(1): 561-574, 2021 01.
Artículo en Inglés | MEDLINE | ID: mdl-33210833

RESUMEN

pH-magnetic dual-responsive nanocomposites have been widely used in drug delivery and gene therapy. Recently, a polypseudorotaxane functionalized magnetic nanoparticle (MNP) was developed by synthesizing the magnetic nanoparticles with cyclodextrin (CD) molecules (CDMNP) via polyethylene glycol (PEG) (CDMNP-PEG-CD). The purpose of this study was to explore the antigenicity and immunogenicity of the nanoparticles in vivo prior to their further application explorations. Here, nanoparticles were assessed in vivo for retention, bio-distribution and immuno-reactivity. The results showed that, once administered intravenously, CDMNP-PEG-CD induced a temporary blood monocyte response and was cleared effectively from the body through the urine system in mice. The introduction of ß-CD and PEG/ß-CD polypseudorotaxane on SiO2 magnetic nanoparticles (SOMNP) limited particle intramuscular dispersion after being injected into mouse gastrocnemius muscle (GN), which led to the prolonged local inflammation and muscle toxicity by CDMNP and CDMNP-PEG-CD. In addition, T cells were found to be more susceptible for ß-CD-modified CDMNP; however, polypseudorotaxane modification partially attenuated ß-CD-induced T cell response in the implanted muscle. Our results suggested that CDMNP-PEG-CD nanoparticles or the decomposition components have potential to prime antigen-presenting cells and to break the muscle autoimmune tolerance.


Asunto(s)
Ciclodextrinas/química , Nanopartículas/química , Polietilenglicoles/química , Sistemas de Liberación de Medicamentos , Espectroscopía de Resonancia Magnética
4.
Eur J Clin Invest ; 51(12): e13634, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34152010

RESUMEN

Accumulating evidence has shown that poor oral hygiene is associated with increased risk of cardiometabolic diseases in Western populations. However, its relevance about the relationships in Chinese adults remains unclear. The China Kadoorie Biobank enrolled 512 715 adults aged 30-79 years in China during 2004-2008. Cox regression was used to estimate adjusted hazard ratios (HRs) for each disease associated with measures of oral hygiene. Overall 9.3% of the participants reported rarely or never brushing teeth at baseline. Participants who rarely or never brushed teeth had adjusted HR of 1.12 (95% CI: 1.09, 1.15) for MVE, with similar HRs for stroke (1.08, 1.05-1.12), intracerebral haemorrhage (1.18, 1.11-1.26) and pulmonary heart disease (1.22, 1.13-1.32) compared with those who brushed teeth regularly. Those who did not brush teeth also had increased risk of cancer (1.09, 1.04-1.14), chronic obstructive pulmonary disease (COPD) (1.12, 1.05-1.20), liver cirrhosis (1.25, 1.09-1.44) and all-cause death (1.25, 1.21-1.28) but not type 2 diabetes (0.94, 0.86-1.03) and chronic kidney disease (0.98, 0.81-1.18). Among Chinese adults, we found that poor oral hygiene is associated with higher risks of major vascular disease, cancer, COPD, liver cirrhosis and all-cause deaths, but not type 2 diabetes and chronic kidney disease.


Asunto(s)
Enfermedades Cardiovasculares/epidemiología , Cirrosis Hepática/epidemiología , Mortalidad , Neoplasias/epidemiología , Enfermedad Pulmonar Obstructiva Crónica/epidemiología , Cepillado Dental/estadística & datos numéricos , Adulto , Anciano , Hemorragia Cerebral/epidemiología , China/epidemiología , Diabetes Mellitus Tipo 2/epidemiología , Femenino , Conductas Relacionadas con la Salud , Humanos , Masculino , Persona de Mediana Edad , Higiene Bucal , Modelos de Riesgos Proporcionales , Enfermedad Cardiopulmonar/epidemiología , Insuficiencia Renal Crónica/epidemiología , Factores de Riesgo , Accidente Cerebrovascular/epidemiología
5.
Ecotoxicol Environ Saf ; 198: 110677, 2020 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-32371214

RESUMEN

The volatilization characteristics and risk evaluation of heavy metals (As, Cd, Co ,Cr, Cu, Ni, Pb, Zn, and Ti) during pyrolysis and combustion of rubber waste with or without molecular sieves (MS) were studied. The addition of MS during pyrolysis inhibited the volatilization of As and promoted the volatilization of Ni and Co, while during combustion it inhibited the volatilization of Pb, Zn and promoted the volatilization of Cu. For Cd, Cr, Ni, Zn and Ti, their volatilization rates during pyrolysis were significantly higher than those during combustion, whereas for As and Cu, the volatilization rates during pyrolysis were lower than those during combustion. Risk evaluation of gaseous heavy metals was exhibited based on the Potential Ecological Risk Index (RI) method. The potential ecological risk during combustion was generally lower than that during pyrolysis. The research results provide important information of heavy metals control during waste thermal treatment.


Asunto(s)
Metales Pesados/análisis , Eliminación de Residuos , China , Gases , Pirólisis , Medición de Riesgo , Goma , Volatilización
6.
Anal Bioanal Chem ; 410(28): 7357-7371, 2018 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-30294762

RESUMEN

The co-adsorption of mixed nonionic surfactant and imidazolium-based ionic liquid, Triton X100 (TX100), with 1-cetyl-3-methylimidazolium bromide (C16mimBr) adsorbed onto the surface of magnetic halloysite nanotubes (MHNTs) was used as an efficient adsorbent for simultaneous determination of amlodipine and nimodipine in urine. The designed adsorbent was characterized by TEM, TGA, FTIR, and DLS analysis methods. All the parameters that influence the extraction efficiency are optimized with the aid of response surface methodology (RSM). The effects of nonionic surfactant TX100 with different structures of ionic-liquid-coated MHNTs were investigated. Under optimum conditions, extraction recoveries of amlodipine and nimodipine were in the range of 73.8-81.2 and 94.3-96.1%, with RSDs (n = 3) of 2.6-5.5% in spiked urine samples, respectively. The adsorption mechanism principal of mixed hemimicelles was discussed in this study. The limit of detection obtained for analytes was < 0.002 µg·mL-1. To our knowledge, this was the first attempt using a mixed hemimicelle solid-phase extraction (SPE) based on MHNTs and nonionic surfactant and imidazolium-based ionic liquid for the simultaneous determination of amlodipine and nimodipine in biological samples. Graphical abstract ᅟ.


Asunto(s)
Silicatos de Aluminio/química , Imidazoles/química , Líquidos Iónicos/química , Micelas , Nanotubos/química , Octoxinol/química , Adsorción , Amlodipino/orina , Arcilla , Humanos , Magnetismo , Modelos Moleculares , Estructura Molecular , Nimodipina/orina , Extracción en Fase Sólida/métodos , Tensoactivos/química
7.
Molecules ; 23(10)2018 10 11.
Artículo en Inglés | MEDLINE | ID: mdl-30314388

RESUMEN

Skin wound healing is a complex and dynamic process that involves angiogenesis and growth factor secretion. Newly formed vessels can provide nutrition and oxygen for skin wound healing. Growth factors in skin wounds are important for keratinocytes and fibroblasts proliferation, epithelialization, extracellular matrix remodeling, and angiogenesis, which accelerate skin wound healing. Therefore, treatment strategies that enhance angiogenesis and growth factors secretion in skin wounds can accelerate skin wound healing. This study investigated the effects of a SIKVAV (Ser-Ile-Lys-Val-Ala-Val) peptide-modified chitosan hydrogel on skin wound healing. Hematoxylin and eosin (H&E) staining demonstrated that the SIKVAV-modified chitosan hydrogel accelerated the re-epithelialization of wounds compared with that seen in the negative and positive controls. Masson's trichrome staining showed that more collagen fibers were deposited in the skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. Immunohistochemistry assays demonstrated that more myofibroblasts were deposited and more angiogenesis occurred in skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. In addition, ELISA assays showed that the SIKVAV-modified chitosan hydrogels promoted the secretion of growth factors in skin wounds. Taken together, these results suggest that the SIKVAV-modified chitosan hydrogel has the potential to be developed as synthesized biomaterials for the treatment of skin wounds.


Asunto(s)
Materiales Biocompatibles/química , Quitosano/química , Hidrogeles/química , Oligopéptidos/química , Piel Artificial , Cicatrización de Heridas , Animales , Materiales Biocompatibles/síntesis química , Colágeno/biosíntesis , Fibroblastos/metabolismo , Hidrogeles/síntesis química , Péptidos y Proteínas de Señalización Intercelular/biosíntesis , Ratones , Neovascularización Fisiológica , Repitelización
8.
Biochem J ; 472(1): 55-69, 2015 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-26349540

RESUMEN

Oculodentodigital dysplasia (ODDD) is primarily an autosomal dominant disorder linked to over 70 GJA1 gene [connexin43 (Cx43)] mutations. For nearly a decade, our laboratory has been investigating the relationship between Cx43 and ODDD by expressing disease-linked mutants in reference cells, tissue-relevant cell lines, 3D organ cultures and by using genetically modified mouse models of human disease. Although salient features of Cx43 mutants have been revealed, these models do not necessarily reflect the complexity of the human context. To further overcome these limitations, we have acquired dermal fibroblasts from two ODDD-affected individuals harbouring D3N and V216L mutations in Cx43, along with familial controls. Using these ODDD patient dermal fibroblasts, which naturally produce less GJA1 gene product, along with RNAi and RNA activation (RNAa) approaches, we show that manipulating Cx43 expression triggers cellular gene reprogramming. Quantitative RT-PCR, Western blot and immunofluorescent analysis of ODDD patient fibroblasts show unusually high levels of extracellular matrix (ECM)-interacting proteins, including integrin α5ß1, matrix metalloproteinases as well as secreted ECM proteins collagen-I and laminin. Cx43 knockdown in familial control cells produces similar effects on ECM expression, whereas Cx43 transcriptional up-regulation using RNAa decreases production of collagen-I. Interestingly, the enhanced levels of ECM-associated proteins in ODDD V216L fibroblasts is not only a consequence of increased ECM gene expression, but also due to an apparent deficit in collagen-I secretion which may further contribute to impaired collagen gel contraction in ODDD fibroblasts. These findings further illuminate the altered function of Cx43 in ODDD-affected individuals and highlight the impact of manipulating Cx43 expression in human cells.


Asunto(s)
Conexina 43/genética , Anomalías Craneofaciales/genética , Anomalías del Ojo/genética , Fibroblastos/metabolismo , Deformidades Congénitas del Pie/genética , Regulación de la Expresión Génica , Sindactilia/genética , Anomalías Dentarias/genética , Animales , Técnicas de Cultivo de Célula , Células Cultivadas , Conexina 43/metabolismo , Anomalías Craneofaciales/metabolismo , Anomalías Craneofaciales/patología , Dermis/patología , Modelos Animales de Enfermedad , Anomalías del Ojo/metabolismo , Anomalías del Ojo/patología , Deformidades Congénitas del Pie/metabolismo , Deformidades Congénitas del Pie/patología , Humanos , Immunoblotting , Ratones , Microscopía Confocal , Mutación , Interferencia de ARN , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sindactilia/metabolismo , Sindactilia/patología , Anomalías Dentarias/metabolismo , Anomalías Dentarias/patología
9.
J Cell Sci ; 126(Pt 13): 2857-66, 2013 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-23606748

RESUMEN

Oculodentodigital dysplasia (ODDD) is mainly an autosomal dominant human disease caused by mutations in the GJA1 gene, which encodes the gap junction protein connexin43 (Cx43). Surprisingly, there have been two autosomal recessive mutations reported that cause ODDD: a single amino acid substitution (R76H) and a premature truncation mutation (R33X). When expressed in either gap junctional intercellular communication (GJIC)-deficient HeLa cells or Cx43-expressing NRK cells, the R76H mutant trafficked to the plasma membrane to form gap junction-like plaques, whereas the R33X mutant remained diffusely localized throughout the cell, including the nucleus. As expected, the R33X mutant failed to form functional channels. In the case of the R76H mutant, dye transfer studies in HeLa cells and electrical conductance analysis in GJIC-deficient N2a cells revealed that this mutant could form functional gap junction channels, albeit with reduced macroscopic and single channel conductance. Alexa 350 dye transfer studies further revealed that the R76H mutant had no detectable negative effect on the function of co-expressed Cx26, Cx32, Cx37 or Cx40, whereas the R33X mutant exhibited significant dominant or trans-dominant effects on Cx43 and Cx40 as manifested by a reduction in wild-type connexin gap junction plaques. Taken together, our results suggest that the trans-dominant effect of R33X together with its complete inability to form a functional channel may explain why patients harboring this autosomal recessive R33X mutant exhibit greater disease burden than patients harboring the R76H mutant.


Asunto(s)
Sustitución de Aminoácidos , Codón sin Sentido , Conexina 43/metabolismo , Anomalías Craneofaciales/metabolismo , Anomalías del Ojo/metabolismo , Deformidades Congénitas del Pie/metabolismo , Sindactilia/metabolismo , Anomalías Dentarias/metabolismo , Línea Celular Tumoral , Membrana Celular/metabolismo , Conexina 26 , Conexina 43/genética , Conexinas , Anomalías Craneofaciales/genética , Anomalías Craneofaciales/patología , Citosol/metabolismo , Anomalías del Ojo/genética , Anomalías del Ojo/patología , Colorantes Fluorescentes/metabolismo , Deformidades Congénitas del Pie/genética , Deformidades Congénitas del Pie/patología , Uniones Comunicantes/metabolismo , Uniones Comunicantes/patología , Genes Recesivos , Humanos , Microscopía Fluorescente , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Transporte de Proteínas , Sindactilia/genética , Sindactilia/patología , Anomalías Dentarias/genética , Anomalías Dentarias/patología
10.
Can J Physiol Pharmacol ; 93(8): 597-602, 2015 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-26047259

RESUMEN

This study examined the effects of polyethylene oxide (PEO) on the survival rate, hemodynamics, blood gas indexes, lactic acid levels, microcirculation, and inflammatory cytokine levels in rats subjected to severe hemorrhagic shock. The shocked rats were resuscitated with either Ringer's lactate solution or 20 ppm of PEO in Ringer's lactate solution for 1 h. It was found that infusion of PEO effectively improved the survival, metabolic acidosis, oxygen delivery, hyperlactacidemia, tissue perfusion, and inflammatory responses of rats subjected to hemorrhagic shock. In addition, we found, for the first time, that PEO showed protective effects on hepatic and renal injury, as evidenced by the significant decreases in the elevated levels of alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, and creatinine caused by shock induction after infusion of PEO (p < 0.05, 60 min post-resuscitation by comparison with pre-resuscitation). All of these findings indicate that PEO exhibits strong therapeutic effects under conditions of severe hemorrhagic shock,which also provides theoretical and experimental bases for the clinical use of PEO.


Asunto(s)
Riñón/efectos de los fármacos , Fallo Hepático/prevención & control , Hígado/efectos de los fármacos , Insuficiencia Multiorgánica/prevención & control , Polietilenglicoles/administración & dosificación , Sustancias Protectoras/administración & dosificación , Insuficiencia Renal/prevención & control , Choque Hemorrágico/terapia , Animales , Biomarcadores/sangre , Modelos Animales de Enfermedad , Fluidoterapia/métodos , Hemodinámica/efectos de los fármacos , Mediadores de Inflamación/sangre , Infusiones Intravenosas , Soluciones Isotónicas/administración & dosificación , Riñón/metabolismo , Riñón/fisiopatología , Ácido Láctico/sangre , Hígado/metabolismo , Hígado/fisiopatología , Fallo Hepático/sangre , Fallo Hepático/etiología , Fallo Hepático/fisiopatología , Masculino , Microcirculación/efectos de los fármacos , Insuficiencia Multiorgánica/etiología , Insuficiencia Multiorgánica/fisiopatología , Ratas Sprague-Dawley , Insuficiencia Renal/sangre , Insuficiencia Renal/etiología , Insuficiencia Renal/fisiopatología , Lactato de Ringer , Índice de Severidad de la Enfermedad , Choque Hemorrágico/sangre , Choque Hemorrágico/complicaciones , Choque Hemorrágico/fisiopatología , Factores de Tiempo
11.
Biochem J ; 457(3): 441-9, 2014 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-24228978

RESUMEN

To date, over 65 mutations in the gene encoding Cx43 (connexin43) have been linked to the autosomal-dominant disease ODDD (oculodentodigital dysplasia). A subset of these patients experience bladder incontinence which could be due to underlying neurogenic deterioration or aberrant myogenic regulation. BSMCs (bladder smooth muscle cells) from wild-type and two Cx43 mutant lines (Cx43(G60S) and Cx43(I130T)) that mimic ODDD exhibit a significant reduction in total Cx43. Dye transfer studies revealed that the G60S mutant was a potent dominant-negative inhibitor of co-expressed Cx43, a property not equally shared by the I130T mutant. BSMCs from both mutant mouse strains were defective in their ability to contract, which is indicative of phenotype changes due to harbouring the Cx43 mutants. Upon stretching, Cx43 levels were significantly elevated in controls and mutants containing BSMCs, but the non-muscle myosin heavy chain A levels were only reduced in cells from control mice. Although the Cx43(G60S) mutant mice showed no difference in voided urine volume or frequency, the Cx43(I130T) mice voided less frequently. Thus, similar to the diversity of morbidities seen in ODDD patients, genetically modified mice also display mutation-specific changes in bladder function. Furthermore, although mutant mice have compromised smooth muscle contraction and response to stretch, overriding bladder defects in Cx43(I130T) mice are likely to be complemented by neurogenic changes.


Asunto(s)
Conexina 43/metabolismo , Anomalías Craneofaciales/fisiopatología , Modelos Animales de Enfermedad , Anomalías del Ojo/fisiopatología , Deformidades Congénitas del Pie/fisiopatología , Músculo Liso/fisiopatología , Enfermedades Musculares/etiología , Sindactilia/fisiopatología , Anomalías Dentarias/fisiopatología , Vejiga Urinaria Neurogénica/etiología , Vejiga Urinaria/fisiopatología , Sustitución de Aminoácidos , Animales , Comunicación Celular , Células Cultivadas , Conexina 43/antagonistas & inhibidores , Conexina 43/genética , Anomalías Craneofaciales/metabolismo , Anomalías Craneofaciales/patología , Anomalías del Ojo/metabolismo , Anomalías del Ojo/patología , Deformidades Congénitas del Pie/metabolismo , Deformidades Congénitas del Pie/patología , Uniones Comunicantes/metabolismo , Masculino , Ratones , Ratones Mutantes , Contracción Muscular , Músculo Liso/química , Músculo Liso/metabolismo , Músculo Liso/patología , Enfermedades Musculares/fisiopatología , Proteínas Mutantes/antagonistas & inhibidores , Proteínas Mutantes/metabolismo , Cadenas Pesadas de Miosina/metabolismo , Sindactilia/metabolismo , Sindactilia/patología , Anomalías Dentarias/metabolismo , Anomalías Dentarias/patología , Vejiga Urinaria/química , Vejiga Urinaria/metabolismo , Vejiga Urinaria/patología , Vejiga Urinaria Neurogénica/fisiopatología , Incontinencia Urinaria/etiología
12.
BMC Public Health ; 14: 422, 2014 May 03.
Artículo en Inglés | MEDLINE | ID: mdl-24884431

RESUMEN

BACKGROUND: This study was conducted to ascertain the feasibility of using rapid oral fluid testing as an alternative HIV testing method in China. METHOD: This is a mixed-method study among men who have sex with men (MSM), female sex workers (FSW) and VCT clients, conducted in 4 cities in Shandong Province. A pre-tested questionnaire was administered to 1137 participants through face-to-face interview to assess demographic characteristics, HIV testing histories and willingness to accept rapid oral fluid testing. VCT clients were provided with the saliva test kits for a screening test and errors in operation were recorded. Testing results were compared between oral and blood testing. Short feedback questionnaire was administered to 200 FSW who had undergone oral testing. RESULTS: The rate of willingness to take oral-fluid HIV testing among MSM, FSW and VCT clients was 72.8%, 72.1% and 67.4% respectively. Common errors recorded during test kit operation by the 229 VCT clients included: unpreparedness, wrong swab sampling, wrong dilution, wrong testing and inability to read test results. Advantages of oral testing listed by participants included: less intrusive, painlessness, easy self- testing and privacy. Disadvantages included perceived unreliable results (55.5%) and not nationally recognised (9%). Comparison of saliva and the blood testing results recorded a consistency rate of 0.970 (χ2 = 153.348, P < 0.001), implying an excellent consistency. CONCLUSION: Introduction of oral rapid fluid testing as an alternative HIV testing method in China is highly feasible but with some challenges including low recognition and operation errors.


Asunto(s)
Infecciones por VIH/diagnóstico , Infecciones por VIH/metabolismo , Tamizaje Masivo/métodos , Saliva/metabolismo , Sexo Inseguro/estadística & datos numéricos , Adolescente , Adulto , China , Estudios de Factibilidad , Femenino , Homosexualidad Masculina/estadística & datos numéricos , Humanos , Masculino , Tamizaje Masivo/estadística & datos numéricos , Reproducibilidad de los Resultados , Trabajadores Sexuales/estadística & datos numéricos , Encuestas y Cuestionarios , Adulto Joven
13.
Front Immunol ; 15: 1365118, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38545121

RESUMEN

Background: The association between psoriasis vulgaris and bullous pemphigoid (BP) remains largely unknown. Objectives: To investigate whether there is a causal effect between psoriasis vulgaris and BP. Methods: Two-sample bidirectional Mendelian randomization (MR) analyses were conducted using publicly released genome-wide association studies (GWAS) summary statistics. The GWAS summary statistics for BP were downloaded online from FinnGen Biobank Documentation of the R12 release, which includes 219 BP cases and 218,066 controls. The GWAS data for psoriasis vulgaris were extracted from Sakaue et al., which comprises 5072 cases and 478,102 controls. Single-nucleotide polymorphisms (SNPs) associated with exposure were selected as instrumental variables by performing additional quality control steps. The inverse-variance-weighted (IVW) method was used for the primary MR analyses, and the MR-Egger regression, weighted mode method, weighted median method, and simple mode were employed for sensitivity analyses. The MR-Egger intercept test and "leave-one-out" sensitivity analysis were performed to evaluate the horizontal pleiotropy and the potentially influential SNPs, respectively. Results: Genetically determined log odds of psoriasis vulgaris were associated with an increased risk of BP (IVW: odds ratio (OR) = 1.263, 95% confidence interval (CI): 1.013-1.575, P=0.038). Sensitivity analyses by the weighted mode (OR=1.255, 95%CI: 0.973-1.618, P=0.106), MR Egger (OR=1.315, 95%CI: 0.951-1.817, P=0.126), simple mode (OR=1.414, 95%CI: 0.823-2.429, P=0.234) and weighted median method (OR=1.177, 95%CI: 0.889-1.559, P=0.254) derived directionally consistent relationship between the genetically predicted log odds of psoriasis vulgaris and risks of developing BP. On the contrary, we found that genetically predicted BP had no significant effect on psoriasis vulgaris (IVW: OR=0.996, P= 0.707), indicating the unidirectionality of the relationship. MR-Egger intercept tests showed no evidence of horizontal pleiotropy. No influential SNP driving the results was detected by the leave-one-out sensitivity analysis. Conclusions: Our results suggested that psoriasis vulgaris causally increases the risk of BP, highlighting the need for potential strategies for the prevention and early diagnosis of comorbid BP in patients with psoriasis vulgaris. Further researches into this association and underlying mechanisms are warranted.


Asunto(s)
Penfigoide Ampolloso , Psoriasis , Estilbenos , Humanos , Estudio de Asociación del Genoma Completo , Análisis de la Aleatorización Mendeliana , Penfigoide Ampolloso/genética , Psoriasis/genética , Nonoxinol
14.
Shanghai Kou Qiang Yi Xue ; 33(2): 175-179, 2024 Apr.
Artículo en Zh | MEDLINE | ID: mdl-39005095

RESUMEN

PURPOSE: To explore the therapeutic effect of stromal cell derived factor-1α(SDF-1α) combined with platelet-poor plasma(PPP) on permanent tooth avulsion injury. METHODS: One hundred and forty-four patients with permanent tooth avulsion injuries admitted to Hengshui People's Hospital from March 2020 to March 2022, with a total of 152 affected teeth were included. They were randomly divided into experimental group of 72 cases(76 teeth) and control group of 72 cases(76 teeth). The control group underwent routine replantation surgery, and the root tips were soaked and rinsed with PPP biological solution before surgery. On the basis of the control group, the experimental group implanted SDF-1α into the alveolar fossa before in vitro tooth implantation for treatment. The patients were followed up for 12 months after surgery, the success rate of implantation, degree of postoperative occlusal pain, expression of inflammatory factors in gingival fluid, serum growth factor expression, and incidence of postoperative complications were compared between the two groups. Statistical analysis was performed with SPSS 19.0 software package. RESULTS: The success rate of replantation in the control group was 90.79%(69/76), while the experimental group was 98.68%(75/76). The success rate of replantation in the experimental group was significantly higher than that in the control group(P<0.05). On the 2nd day, 3 months and 12 months after surgery, the pain scores of the two groups of patients gradually decreased (P<0.05). There was no significant difference in pain scores between the two groups at each time point(P>0.05). One month after surgery, the CRP and IL-6 levels in both groups decreased(P<0.05), while the experimental group was significantly lower than the control group(P<0.05). The soluble intercellular adhesion factor (sICAM-1) in both groups increased, and the experimental group was significantly higher than the control group (P<0.05). One month after surgery, vascular endothelial growth factor(VEGF), fibroblast growth factor(FGF), transforming growth factor beta(TGF-ß)and platelet derived growth factor(PDGF) were all elevated, and the experimental group was significantly higher than the control group(P<0.05). The incidence of postoperative complications in the experimental group was significantly lower than that in the control group(P<0.05). CONCLUSIONS: The combination of PPP and SDF-1α in treating patients with permanent tooth avulsion injury has a high success rate of delayed replantation and a low incidence of postoperative adverse reactions.


Asunto(s)
Quimiocina CXCL12 , Avulsión de Diente , Reimplante Dental , Humanos , Avulsión de Diente/cirugía , Reimplante Dental/métodos , Plasma , Dentición Permanente
15.
Int J Biol Macromol ; 264(Pt 1): 130460, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38437937

RESUMEN

Designing carbon materials with specific oxygen-containing functional groups is very attractive for the precise decoration of carbon electrode materials and the basic understanding of specific charge storage mechanisms, which contributes to the further development of high-performance carbon materials for energy storage and conversion applications. In this contribution, a hydroxyl-rich micropore-dominated porous carbon material was obtained by direct carbonization of cellulose. The content of oxygen atoms in hydroxyl form in the obtained carbon is nearly 6 at.%. With the pyrolysis temperature changed, the macroscopic morphology, the specific surface area, surface functional groups, and graphitization degree of the carbon materials were changed strongly. Besides, the carbon material obtained with a carbonization temperature of 900 °C (C9) showed enhanced specific capacitance in sulfuric acid, sodium hydroxide, and sodium sulfate aqueous electrolytes, which mainly originates from the contribution of pseudocapacitance. The pseudocapacitance mainly depends on the presence of surface hydroxyl functional groups. Besides, the pseudocapacitance value of C9 material in neutral electrolytes (151.34 F g-1) is about twice that in acidic (75.9 F g-1) and alkaline (75.78 F g-1) electrolytes.


Asunto(s)
Celulosa , Radical Hidroxilo , Porosidad , Carbono , Electrólitos , Oxígeno
16.
ACS Appl Bio Mater ; 7(5): 3283-3294, 2024 05 20.
Artículo en Inglés | MEDLINE | ID: mdl-38727030

RESUMEN

Medical implants are constantly facing the risk of bacterial infections, especially infections caused by multidrug resistant bacteria. To mitigate this problem, gold nanoparticles with alkyl bromide moieties (Au NPs-Br) on the surfaces were prepared. Xenon light irradiation triggered the plasmon effect of Au NPs-Br to induce free radical graft polymerization of 2-(dimethylamino)ethyl methacrylate (DMAEMA), leading to the formation of poly(DMAEMA) brush-grafted Au NPs (Au NPs-g-PDM). The Au NPs-g-PDM nanocomposites were conjugated with phytic acid (PA) via electrostatic interaction and van der Waals interaction. The as-formed aggregates were deposited on the titanium (Ti) substrates to form the PA/Au NPs-g-PDM (PAP) hybrid coatings through surface adherence of PA and the gravitational effect. Synergistic bactericidal effects of contact-killing caused by the cationic PDM brushes, and local heating generated by the Au NPs under near-infrared irradiation, conferred strong antibacterial effects on the PAP-deposited Ti (Ti-PAP) substrates. The synergistic bactericidal effects reduced the threshold temperature required for the photothermal sterilization, which in turn minimized the secondary damage to the implant site. The Ti-PAP substrates exhibited 97.34% and 99.97% antibacterial and antiadhesive efficacy, respectively, against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), compared to the control under in vitro antimicrobial assays. Furthermore, the as-constructed Ti-PAP surface exhibited a 99.42% reduction in the inoculated S. aureus under in vivo assays. In addition, the PAP coatings exhibited good biocompatibility in the hemolysis and cytotoxicity assays as well as in the subcutaneous implantation of rats.


Asunto(s)
Antibacterianos , Escherichia coli , Oro , Ensayo de Materiales , Nanopartículas del Metal , Pruebas de Sensibilidad Microbiana , Tamaño de la Partícula , Ácido Fítico , Staphylococcus aureus , Oro/química , Oro/farmacología , Antibacterianos/farmacología , Antibacterianos/química , Antibacterianos/síntesis química , Nanopartículas del Metal/química , Ácido Fítico/química , Ácido Fítico/farmacología , Staphylococcus aureus/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Animales , Propiedades de Superficie , Materiales Biocompatibles Revestidos/química , Materiales Biocompatibles Revestidos/farmacología , Cationes/química , Cationes/farmacología , Polímeros/química , Polímeros/farmacología , Titanio/química , Titanio/farmacología
17.
Macromol Rapid Commun ; 34(23-24): 1827-32, 2013 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-24214231

RESUMEN

The attempts to mediate iterative RAFT polymerization of ionic monomers through visible light irradiation in water at 20 °C is reported, in which complete conversions are attained in several tens of minutes and the propagation suspends/restarts immediately for multiple times on cycling irradiation. This technique suits the one-pot synthesis of NH2 /imidazole-based polymers with tuned structures from homo to random, block, random-block, and block-random-block, thus is robust and promising to control the sequence of the ionized water-soluble reactive copolymers.


Asunto(s)
Luz , Polímeros/química , Agua/química , Imidazoles/química , Polimerizacion , Temperatura
18.
Environ Pollut ; 334: 122121, 2023 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-37385359

RESUMEN

As an alternative to polybrominated diphenyl ethers (PBDEs), decabromodiphenyl ethane (DBDPE) has become one of the most important new brominated flame retardants (NBFRs). However, little is known about whether this emerging contaminant may has an environmental fate similar to PBDEs. Sediments are the main sink for DBDPE in the aqueous phase. Worldwide concentration data, since it was first found in sediments to date, have been collated, and the following conclusions have been drawn. (1) DBDPE concentrations in sediments have increased rapidly, often with a higher risk of contamination in source discharge areas. Compared with other countries, DBDPE contamination in China is more severe, especially in Guangdong Province, which is closely related to its being an e-waste dismantling area. (2) The amount of DBDPE in surface sediments has exceeded that of legacy brominated flame retardants (BFRs), and data recorded in sediment cores also corroborate that DBDPE is replacing decabromodiphenyl ether (BDE-209) as one of the most dominant NBFRs in the environment. (3) The exposure pathways of DBDPE include dietary intake, air or indoor dust intake, cutaneous absorption and endogenous exposure. For sediments, dietary exposure and endogenous exposure pathways need to be considered. Sediment DBDPE can enter the human body through bioenrichment such as contaminated seafood and the food chain. (4) DBDPE can exhibit neurotoxicity, thyrotoxicity, reproductive and developmental toxicity, hepatotoxicity and oxidative stress in organisms. Long-term DBDPE exposure may increase hyperthyroidism risk and inhibit normal cells activity. This review focuses on the distribution characteristics and exposure risks of DBDPE in global water sediments, providing a strong reference for environmental management and related legal policy formulation. The next steps are to focus on continuous source monitoring, process control and sediment clean-up of DBDPE. The development of sustainable water management options for waste microplastics (MPs) and e-waste spiked with DBDPE is a priority.


Asunto(s)
Monitoreo del Ambiente , Retardadores de Llama , Humanos , Retardadores de Llama/toxicidad , Retardadores de Llama/análisis , Éteres Difenilos Halogenados/toxicidad , Éteres Difenilos Halogenados/análisis , Agua , Plásticos
19.
Chemosphere ; 325: 138397, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-36925014

RESUMEN

The feasibility and potential mechanisms of the self-alkali activation brought by municipal solid waste incineration (MSWI) fly ashes to the self-cementation of arsenic-contaminated soils were quantitatively evaluated and comprehensively analyzed to avoid the additional application of the alkali activators and binder materials traditionally. The employment of the two kinds of precursor materials achieved the self-alkali-activated self-cementation ('double self') under ambient conditions. The largest compressive strength (MPa) of 25.64 and lowest leaching toxicities (mg/L) of 21.05, 2.86, 0.08, 0.02, 2.05, and 0.34 for Zn, Cu, Cr, Cd, Pb, and As were obtained in the solidified matrix. Geopolymerization kinetics of the 'double self' cementation can be mathematically fitted by the Johnson-Mehl-Avrami-Kolmogorov model. CaClOH and halite in the MSWI fly ashes set up the self-alkali activation by reacting with the kaolinite and quartz in soils contaminated with arsenic by forming layered hydration and three-dimensional geopolymerization products to push for self-cementation.


Asunto(s)
Arsénico , Metales Pesados , Residuos Sólidos/análisis , Ceniza del Carbón , Incineración/métodos , Álcalis , Cementación , Metales Pesados/análisis
20.
Waste Manag ; 161: 166-177, 2023 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-36889123

RESUMEN

The solidification/stabilization technique recommended for the disposal of municipal solid waste incineration (MSWI) fly ashes in developed countries was inappropriate for the treatment in most developing counterparts. In this study, the diatomite and MoS2 nanosheets were synergistically employed to activate the self-alkali-activated cementation of the MSWI fly ashes to achieve efficient solidification, the immobilization of heavy metals (HMs), and the inhibition of chloride release. The compressive strength of 28.61 MPa and the leaching toxicities (mg/L) of Zn, Pb, Cu, Cd, and Cr of 2.26, 0.87, 0.5, 0.06, and 0.22 were obtained from the hardened mortars. Diatomite significantly influenced the self-alkali-activated cementation of the MSWI fly ashes while MoS2 nanosheets played both roles in intensifying the stabilization of HMs and strengthening the binding process by inducing the formation of sodalite and kaolinite, enhancing the growth rates of nucleation, and transforming the layered cementation to the partial and full three-dimensional cementation in the hardened matrix. This study not only verified the feasibility of diatomite and MoS2 in activating the self-alkali-activated cementation of the MSWI fly ashes but also supplied a reliable technique for the harmless disposal and efficient utilization of MSWI fly ashes in developing countries.


Asunto(s)
Metales Pesados , Eliminación de Residuos , Residuos Sólidos/análisis , Incineración/métodos , Ceniza del Carbón , Eliminación de Residuos/métodos , Molibdeno , Álcalis , Cementación , Material Particulado , Carbono , Metales Pesados/análisis
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