RESUMEN
Polyaniline (PANi) films were prepared by direct polymerizing deposition with four different kinds of acids as dopants or were prepared by a casting method on the surface of a polytetrafluoroethylene substrate. The properties of PANi films were characterized using atomic force microscopy, electrical conductivity measurements, and water contact angle measurements. Unlike the casting PANi film, experimental results indicated that the synthesized PANi films had a similar nanostructure as that of average nanoparticles (approximate diameter of 30-50 nm). To investigate the potential usefulness of PANi films in biomedical applications, we also studied their biocompatibility through the adhesion and proliferation properties of PC-12 pheochromocytoma cells. All the films were found to be biocompatible and allowed cell attachment and proliferation. However, the synthesized films have a much higher ability for cell adhesion than the casting film. After 4 days of culture on different PANi films, the cells formed more confluent monolayers on the synthesized PANi films than on the casting films. These results demonstrate that the PANi films could be used to culture neurotic cells and that their surface architecture on the nanoscale may affect cell function such as attachment and proliferation.
Asunto(s)
Ácidos Acíclicos/química , Ácidos no Carboxílicos/química , Compuestos de Anilina/química , Materiales Biocompatibles/química , Nanoestructuras/química , Andamios del Tejido/química , Compuestos de Anilina/síntesis química , Animales , Materiales Biocompatibles/síntesis química , Adhesión Celular , Proliferación Celular , Forma de la Célula , Ácido Cítrico/química , Conductividad Eléctrica , Ácido Clorhídrico/química , Interacciones Hidrofóbicas e Hidrofílicas , Malatos/química , Microscopía de Fuerza Atómica , Microscopía Electrónica de Rastreo , Nanopartículas/química , Células PC12 , Tamaño de la Partícula , Percloratos/química , Politetrafluoroetileno/química , Ratas , Propiedades de Superficie , HumectabilidadRESUMEN
A new kind of phospholipid product with fluorescence was derived from autoxidation of an aminophospholipid, L-alpha-phosphatidylethanolamine dilinoleoyl in solid film state. Atomic force microscopy showed that the film product was composed of round and elliptical vesicles with diameters of about 20-45 nm. The product was difficult to dissolve in water and most organic solvents. Fourier transform infrared spectroscopy, solid-state (13)C NMR, and solid-state (31)P NMR indicated that the unsaturated double carbon bond decreased with the reaction, and polymerization within/between phospholipid molecules occurred. Endothelial cells from human vein seeded onto the film showed the highest proliferating activity compared with seeding onto Corning culture plate, glass plate, or phospholipid film with phosphorylcholine head group, as evaluated by 3-(4,5-timethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (p < 0.001). The hemocompatibility of the film was also assessed by platelet adhesion and protein adsorption. The results suggest that the product has potential applications as a new biomaterial coating.