A molecularly imprinted electrochemical sensor based on surface imprinted polymerization and boric acid affinity for selective and sensitive detection of P-glycoproteins.

In this study, a molecular imprinted electrochemical sensor based on surface imprinting and boric acid affinity was prepared for the selective and sensitive detection of P-glycoprotein (P-gp) under physiological conditions. Several polymerization parameters were systematically investigated. Four electrochemical methods were used to characterize the sensors. It was proved that the glassy carbon electrode (GCE) was well modified by molecularly imprinted polymers (MIPs), and the electrochemical detection results were consistent with the theory. Methodological verification also showed that there were different linear relationships in the range of 1.0E-10-1.0E-1 µg/mL and 0.5-10 µg/mL with LOD = 2.233E-11 µg/mL and LOQ = 7.444E-11 µg/mL. The excellent selectivity and specificity of the sensor were demonstrated and the sensor could be stored in 1X PBS (0.01 M) at 4 °C for one week. Repeatability and intermediate precision were measured, and the RSD remained below 5%. In addition, the sensor could be reused up to three times without affecting the results of the measurements. The results of application showed that the sensor had a more accurate response value in 1% of plasma matrix. Compared with the detection results of ELISA kit method, the sensor had the advantages of wider detection range, shorter detection time, higher accuracy, simple operation and low cost, which indicated that the sensor had a certain practicability and clinical translational application capability. This study also provided a new idea and method for the detection of other high molecular weight biomarkers in complex biological samples.

H2O2 Self-Supplying and GSH-Depleting Nanoplatform for Chemodynamic Therapy Synergetic Photothermal/Chemotherapy.

Chemodynamic therapy (CDT) that utilizes Fenton-type reactions to convert endogenous hydrogen peroxide (H2O2) into hydroxyl radicals (•OH) is a promising strategy in anticancer treatment, but the overexpression of glutathione (GSH) and limited endogenous H2O2 make the efficiency of CDT unsatisfactory. Here, an intelligent nanoplatform CuO2@mPDA/DOX-HA (CPPDH), which induced the depletion of GSH and the self-supply of H2O2, was proposed. When CPPDH entered tumor cells through the targeting effect of hyaluronic acid (HA), a release of Cu2+ and produced H2O2 were triggered by the acidic environment of lysosomes. Then, the Cu2+ was reduced by GSH to Cu+, and the Cu+ catalyzed H2O2 to produce •OH. The generation of •OH could be distinctly enhanced by the GSH depletion and H2O2 self-sufficiency. Besides, an outstanding photothermal therapy (PTT) effect could be stimulated by NIR irradiation on mesoporous polydopamine (mPDA). Meanwhile, mPDA was an excellent photoacoustic reagent, which could monitor the delivery of nanocomposite materials through photoacoustic (PA) imaging. Moreover, the successful delivery of doxorubicin (DOX) realized the integration of chemotherapy, PTT, and CDT. This strategy could solve the problem of insufficient CDT efficacy caused by the limited H2O2 and overexpression of GSH. This multifunctional nanoplatform may open a broad path for self-boosting CDT and synergistic therapy.